Institute of Vegetables and Flowers, Shandong Key Laboratory of Greenhouse Vegetable Biology, Shandong Branch of National Vegetable Improvement Center, Huanghuai Region Vegetable Scientific Station of Ministry of Agriculture (Shandong), Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
Sci Rep. 2019 Dec 30;9(1):20341. doi: 10.1038/s41598-019-56456-1.
Salt stress is one of the abiotic stresses affecting crop growth and yield. The functional screening and mechanism investigation of the genes in response to salt stress are essential for the development of salt-tolerant crops. Here, we found that OXIDATIVE STRESS 2 (OXS2) was a salinity-induced gene, and the mutant oxs2-1 was hypersensitive to salt stress during seed germination and root elongation processes. In the absence of stress, OXS2 was predominantly localized in the cytoplasm; when the plants were treated with salt, OXS2 entered the nuclear. Further RNA-seq analysis and qPCR identification showed that, in the presence of salt stress, a large number of differentially expressed genes (DEGs) were activated, which contain BOXS2 motifs previously identified as the binding element for AtOXS2. Further ChIP analysis revealed that, under salt stress, OXS2 associated with CA1 and Araport11 directly through binding the BOXS2 containing fragments in the promoter regions. In conclusion, our results indicate that OXS2 is required for salt tolerance in Arabidopsis mainly through associating with the downstream CA1 and Araport11 directly.
盐胁迫是影响作物生长和产量的非生物胁迫之一。对响应盐胁迫的基因进行功能筛选和机制研究，对于培育耐盐作物至关重要。在这里，我们发现 OXIDATIVE STRESS 2（OXS2）是一种盐诱导基因，突变体 oxs2-1 在种子萌发和根伸长过程中对盐胁迫高度敏感。在没有胁迫的情况下，OXS2 主要定位于细胞质中；当植物受到盐处理时，OXS2 进入细胞核。进一步的 RNA-seq 分析和 qPCR 鉴定表明，在盐胁迫下，大量差异表达基因（DEGs）被激活，其中包含先前鉴定为 AtOXS2 结合元件的 BOXS2 基序。进一步的 ChIP 分析表明，在盐胁迫下，OXS2 通过直接结合启动子区域中包含 BOXS2 的片段与 CA1 和 Araport11 直接结合。总之，我们的结果表明，OXS2 主要通过直接与下游的 CA1 和 Araport11 结合来参与拟南芥的耐盐性。
