The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China.
The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China.
Prev Vet Med. 2023 Apr;213:105856. doi: 10.1016/j.prevetmed.2023.105856. Epub 2023 Jan 23.
Salmonella and ESBL-producing E. coli pose a threat to public health through the food chain. A cross-sectional study was conducted to determine the prevalence and antimicrobial resistance of Salmonella and ESBL E. coli in apparently healthy lactating dairy cattle in Henan Province. Thirty-five lactating cows per farm were sampled by fecal swabbing from 38 farms, with samples being pooled to a total of 7 pooled samples per herd. Eight of the 266 pooled fecal samples (3.0%) were positive for Salmonella (95% confidence intervals (CI): 1.3, 5.8) with a herd-level Salmonella prevalence of 13.2% (95% CI: 4.4, 28.1). The within-herd prevalence for pooled samples for Salmonella ranged from 0.0% to 28.6%. A high proportion of resistance to tetracycline (6/8) and florfenicol (6/8) was obtained in the cultured Salmonella. Multi-drug resistant isolates were observed on 4/5 Salmonella-positive farms. ESBL E. coli were identified on all farms (100% - 34/34, 95% CI: 89.7, 100). All ESBL E. coli isolates (n = 216) contained the bla gene and two isolates also contained the bla gene. Our study reports the prevalence and antimicrobial resistance of Salmonella and ESBL E. coli in apparently healthy lactating dairy cows from Henan Province.
沙门氏菌和产 ESBL 的大肠杆菌通过食物链对公共健康构成威胁。本研究采用横断面研究方法，旨在确定河南省健康泌乳奶牛中沙门氏菌和产 ESBL 大肠杆菌的流行情况和耐药性。每个农场采集 35 头泌乳奶牛的粪便拭子，共采集了 38 个农场的 266 份粪便样本，每份粪便样本均混合成 7 份混合样本。266 份粪便混合样本中，有 8 份（3.0%）为沙门氏菌阳性（95%置信区间：1.3，5.8），总体沙门氏菌流行率为 13.2%（95%置信区间：4.4，28.1）。混合样本中沙门氏菌的个体内流行率为 0.0%至 28.6%。从分离出的沙门氏菌中，我们发现了较高比例的四环素（6/8）和氟苯尼考（6/8）耐药性。在 4/5 个分离出沙门氏菌的阳性农场中观察到了多重耐药分离株。所有农场均检测到产 ESBL 大肠杆菌（100% - 34/34，95%置信区间：89.7，100）。所有 ESBL 大肠杆菌分离株（n=216）均含有 bla 基因，其中 2 个分离株还含有 bla 基因。本研究报告了河南省健康泌乳奶牛中沙门氏菌和产 ESBL 大肠杆菌的流行情况和耐药性。
