The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 Western Australia, Australia; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China.
The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China.
Prev Vet Med. 2023 Mar;212:105822. doi: 10.1016/j.prevetmed.2022.105822. Epub 2022 Dec 20.
Dairy cattle and their products have been linked to human outbreaks of Salmonella and Escherichia coli (E. coli). The objective of this study was to estimate the prevalence and antimicrobial resistance of Salmonella and to enumerate Extended-spectrum β-lactamase (ESBL)-producing E. coli in apparently healthy lactating dairy cows in Hubei Province, China. In a cross-sectional study, a total of 291 adult lactating dairy cows from 10 dairy farms were sampled for the detection of Salmonella and ESBL E. coli. Overall, Salmonella was cultured from 15 out of 291 sampled animals (5.2 %; 95 % confidence intervals (CI): 2.9, 8.4), all from two herds with a herd prevalence of 20.0 % (95 % CI: 2.5, 55.6) and the main serotype being S. Dublin. The within-herd prevalence ranged between 0.0 % and 33.3 %. ESBL E. coli was detected by culture in all farms with an animal level prevalence of 59.1 % (95 % CI: 53.2, 64.8) and 116 samples (39.9 %, 95 % CI: 34.2, 45.7) contained ESBL E. coli with a number exceeding 10 CFU/g feces. Sixty percent (9/15) of Salmonella isolates were resistant to ampicillin, however all isolates were sensitive to the other 8 antimicrobials tested. Ninety percent (95 % CI: 84.6, 94.1) of ESBL E. coli contained the resistance gene bla, but no ESBL Salmonella was found. Our findings contribute to the understanding of the prevalence and antimicrobial resistance of Salmonella and the enumeration of ESBL E. coli and will assist in the decision-making for the control of Salmonella in Hubei Province.
奶牛及其产品与人类沙门氏菌和大肠杆菌（E. coli）感染爆发有关。本研究旨在估计湖北省健康泌乳奶牛中沙门氏菌的流行率和抗生素耐药性，并对产超广谱β-内酰胺酶（ESBL）大肠杆菌进行计数。在一项横断面研究中，从 10 个奶牛场采集了 291 头成年泌乳奶牛的样本，以检测沙门氏菌和 ESBL 大肠杆菌。总的来说，从 291 头采样动物中培养出了 15 株沙门氏菌（5.2%；95%置信区间（CI）：2.9，8.4），全部来自两个畜群，畜群患病率为 20.0%（95%CI：2.5，55.6），主要血清型为都柏林沙门氏菌。每个畜群内的患病率在 0.0%至 33.3%之间。所有农场均通过培养检测到 ESBL 大肠杆菌，动物水平患病率为 59.1%（95%CI：53.2，64.8），116 份样本（39.9%；95%CI：34.2，45.7）中含有 ESBL 大肠杆菌，粪便中超过 10CFU/g 的数量。60%（15/25）的沙门氏菌分离株对氨苄西林耐药，但所有分离株对其余 8 种测试抗生素均敏感。90%（95%CI：84.6，94.1）的 ESBL 大肠杆菌含有 bla 耐药基因，但未发现 ESBL 沙门氏菌。我们的研究结果有助于了解湖北省沙门氏菌的流行率和抗生素耐药性，以及 ESBL 大肠杆菌的计数情况，并为湖北省控制沙门氏菌提供决策依据。
