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关于在尸检材料中探索新型冠状病毒肺炎的技术说明。

Technical note on the exploration of COVID-19 in autopsy material.

作者信息

Humphries Matthew Phillip, Bingham Victoria, Abdullah Sidi Fatima, Craig Stephanie, Lara Beatrize, El-Daly Hesham, O'Doherty Nicole, Maxwell Perry, Lewis Claire, McQuaid Stephen, Lyness James, James Jacqueline, Snead David R J, Salto-Tellez Manuel

机构信息

Precision Medicine Center of Excellence, Queen's University Belfast, Belfast, UK.

National Pathology Imaging Cooperative, Leeds Teaching Hospitals NHS Trust, Leeds, UK.

出版信息

J Clin Pathol. 2023 Jun;76(6):418-423. doi: 10.1136/jcp-2022-208525. Epub 2023 Jan 30.

DOI:10.1136/jcp-2022-208525
PMID:36717223
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10313984/
Abstract

Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF.

摘要

对新型冠状病毒肺炎患者尸检材料中的免疫反应进行研究可能具有重要意义。我们旨在描述一种经过验证的方案，用于使用多重免疫荧光（mIF）探索新型冠状病毒肺炎的分子病理生理学。我们实验室最初在肿瘤学背景下开发的用于检测组织中新型冠状病毒的经过验证的检测方法，被用于在蛋白质和mRNA水平绘制适应性免疫反应的拓扑结构和复杂性。通过蛋白质或mRNA可在原位检测到新型冠状病毒，其敏感性可能部分与疾病阶段有关。在福尔马林固定、石蜡包埋的肺炎材料中，多重免疫荧光面板强大、可靠且可量化，能够检测与病理过程相关的炎症的拓扑变化。临床尸检对于理解未知/复杂病理生理学的疾病具有重要意义。特别是，尸检材料适用于检测新型冠状病毒，并使用mIF对基于组织的复杂免疫反应进行拓扑描述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/95c983dd38e0/jcp-2022-208525f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/8394375882f0/jcp-2022-208525f01.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/5e583173e402/jcp-2022-208525f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/ef3dd429abc0/jcp-2022-208525f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/1c8d69f4a96d/jcp-2022-208525f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/95c983dd38e0/jcp-2022-208525f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/8394375882f0/jcp-2022-208525f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/da425d573092/jcp-2022-208525f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/5e583173e402/jcp-2022-208525f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/ef3dd429abc0/jcp-2022-208525f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/1c8d69f4a96d/jcp-2022-208525f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d7d/10313984/95c983dd38e0/jcp-2022-208525f06.jpg

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本文引用的文献

1
PD-L1 Multiplex and Quantitative Image Analysis for Molecular Diagnostics.用于分子诊断的PD-L1多重定量图像分析
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2
Tissue-Specific Immunopathology in Fatal COVID-19.COVID-19 致死患者的组织特异性免疫病理学。
Am J Respir Crit Care Med. 2021 Jan 15;203(2):192-201. doi: 10.1164/rccm.202008-3265OC.
3
Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection.用于诊断SARS-CoV-2感染的快速即时护理抗原检测和基于分子的检测
Cochrane Database Syst Rev. 2020 Aug 26;8(8):CD013705. doi: 10.1002/14651858.CD013705.
4
A robust multiplex immunofluorescence and digital pathology workflow for the characterisation of the tumour immune microenvironment.一种稳健的多重免疫荧光和数字病理学工作流程，用于肿瘤免疫微环境的特征分析。
Mol Oncol. 2020 Oct;14(10):2384-2402. doi: 10.1002/1878-0261.12764. Epub 2020 Sep 1.
5
The adaptive immune and immune checkpoint landscape of neoadjuvant treated esophageal adenocarcinoma using digital pathology quantitation.基于数字病理定量分析的新辅助治疗食管腺癌的适应性免疫和免疫检查点景观。
BMC Cancer. 2020 Jun 1;20(1):500. doi: 10.1186/s12885-020-06987-y.
6
Reduction and Functional Exhaustion of T Cells in Patients With Coronavirus Disease 2019 (COVID-19).新型冠状病毒病（COVID-19）患者 T 细胞减少和功能耗竭。
Front Immunol. 2020 May 1;11:827. doi: 10.3389/fimmu.2020.00827. eCollection 2020.
7
Improving the Diagnostic Accuracy of the PD-L1 Test with Image Analysis and Multiplex Hybridization.通过图像分析和多重杂交提高PD-L1检测的诊断准确性。
Cancers (Basel). 2020 Apr 29;12(5):1114. doi: 10.3390/cancers12051114.
8
Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model.新冠病毒、中东呼吸综合征病毒和严重急性呼吸综合征病毒在非人灵长类动物模型中的比较发病机制。
Science. 2020 May 29;368(6494):1012-1015. doi: 10.1126/science.abb7314. Epub 2020 Apr 17.
9
A Call to Action: The Need for Autopsies to Determine the Full Extent of Organ Involvement Associated With COVID-19.行动呼吁：需要进行尸检以确定与 COVID-19 相关的器官受累的全部程度。
Chest. 2020 Jul;158(1):43-44. doi: 10.1016/j.chest.2020.03.060. Epub 2020 Apr 10.
10
QuPath: Open source software for digital pathology image analysis.QuPath：用于数字病理学图像分析的开源软件。
Sci Rep. 2017 Dec 4;7(1):16878. doi: 10.1038/s41598-017-17204-5.