Gómez Leiva M A, Aragón-Ortiz F
Depto. de Bioquímica, Universidad de Costa Rica.
Rev Biol Trop. 1986 Jun;34(1):49-53.
Lachesis muta snake venom induced aggregation of bromelain sensitized human erythrocytes at a concentration of 1 mg/ml. The hemagglutinating protein was purified by DEAE-Sephadex A-50 column chromatography. Polyacrylamide gel electrophoresis revealed at least three bands, whereas SDS electrophoresis in the presence of 2-mercaptoethanol showed a single one. Isoelectric focusing revealed hemagglutinating activity in the range of pH 3-8. The maximum peak (mutina) at pH 5.5. This fraction was active in agglutinating human RBC of types A, B, O Rh (+) and B, O Rh (-). One mM EDTA and 1 mM Ca++ did not alter the agglutinating time significantly. Lactose and inositol inhibited the agglutination of A, B, O Rh (+) and B, O Rh (-) human RBC. The present study showed the non specificity of the hemagglutinating activity of mutina. It was also shown that mutina is a non-mitogenic protein.
1毫克/毫升浓度的矛头蝮蛇毒可诱导菠萝蛋白酶致敏的人红细胞聚集。通过DEAE-葡聚糖A-50柱色谱法纯化血凝蛋白。聚丙烯酰胺凝胶电泳显示至少三条带，而在2-巯基乙醇存在下的SDS电泳显示一条带。等电聚焦显示在pH 3-8范围内有血凝活性。最大峰（mutina）在pH 5.5处。该组分对A、B、O Rh（+）型和B、O Rh（-）型人红细胞有凝集活性。1 mM EDTA和1 mM Ca++对凝集时间无明显影响。乳糖和肌醇抑制A、B、O Rh（+）型和B、O Rh（-）型人红细胞的凝集。本研究表明mutina的血凝活性具有非特异性。还表明mutina是一种非促有丝分裂蛋白。
