Gao Shanshan, Cui Menghua, Li Ruiru, Liang Ling, Liu Ying, Xie Liming
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; University of Chinese Academy of Science, Beijing 100049, China.
Sci Bull (Beijing). 2017 Jan 15;62(1):9-15. doi: 10.1016/j.scib.2016.11.006. Epub 2016 Dec 10.
Fluorescence correlation spectroscopy (FCS) is a widely used method for measuring molecular diffusion and chemical kinetics. However, when a mixture of fluorescent species is taken into account, the conventional FCS method has limitations in extracting autocorrelations for different species and cross correlations between different species. Recently developed fluorescence lifetime correlation spectroscopy (FLCS) based on time-tagged time-resolved (TTTR) photon recording, which can record the global and micro arrival time for each individual photon, has been used to discriminate different species according to fluorescence lifetime. Here, based on two-dimensional lifetime decay maps constructed from TTTR photon stream, we have developed a quantitative lifetime-deconvolution FCS model (LDFCS) to extract precise chemical rates for chemical conversions in multi-species systems. The key point of LDFCS model is separation of different species according to the global distribution of fluorescence lifetime and then deconvolution of autocorrelations and cross-correlations from the two-dimensional lifetime decay maps constructed by the micro arrival times of photon pairs at each delay time.
荧光相关光谱法（FCS）是一种广泛用于测量分子扩散和化学动力学的方法。然而，当考虑荧光物种的混合物时，传统的FCS方法在提取不同物种的自相关以及不同物种之间的交叉相关方面存在局限性。最近基于时间标记时间分辨（TTTR）光子记录开发的荧光寿命相关光谱法（FLCS），它可以记录每个光子的全局和微观到达时间，已被用于根据荧光寿命区分不同物种。在此，基于从TTTR光子流构建的二维寿命衰减图，我们开发了一种定量寿命反卷积FCS模型（LDFCS），以提取多物种系统中化学转化的精确化学速率。LDFCS模型的关键点是根据荧光寿命的全局分布分离不同物种，然后从由每个延迟时间光子对的微观到达时间构建的二维寿命衰减图中反卷积自相关和交叉相关。
