Marine Science & Technology Institute, College of Environmental Science & Engineering, Yangzhou University, Yangzhou City, Jiangsu Province, People's Republic of China.
Key Laboratory of Marine Drugs, the Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, People's Republic of China.
Nat Prod Res. 2024 Jul;38(13):2252-2257. doi: 10.1080/14786419.2023.2171401. Epub 2023 Jan 30.
Four prenylated indole alkaloids (-) were targeted isolated from the mangrove rhizosphere soil-derived fungus HK1-6 by using molecular networking strategies. Among them, the planar structure and relative configuration of notoamide X () were elucidated by detailed analysis of the spectroscopic data especially the NOESY spectrum for the first time and its absolute configuration was determined by ECD spectrum. Furthermore, curated molecular networks of MS/MS data were generated with GNPS which allowed highlighting six prenylated indole alkaloids (, , , , , ) that had not previously been identified in this fungus and two (, ) that had never been observed in any fungus. The MS/MS fragmentation pathway of these prenylated indole alkaloids was summarized.
四种倍半萜吲哚生物碱（-）通过分子网络策略从红树林根际土壤来源的真菌 HK1-6 中靶向分离得到。其中，notoamide X（）的平面结构和相对构型通过详细分析光谱数据，特别是首次进行的 NOESY 光谱得到阐明，其绝对构型通过 ECD 光谱确定。此外，通过 GNPS 生成了 MS/MS 数据的经过整理的分子网络，突出显示了以前在该真菌中未鉴定出的六种倍半萜吲哚生物碱（，，，，，）和两种（，）从未在任何真菌中观察到。总结了这些倍半萜吲哚生物碱的 MS/MS 断裂途径。
