Tangsuksant Thanin, Ummartyotin Sarute, Pongprayoon Thirawudh, Arpornmaeklong Premjit, Apinyauppatham Komsan
Master of Science Program in Dental Implantology, Faculty of Dentistry, Thammasat University Rangsit Campus, Khlong Luang, Thailand.
Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University Rangsit Campus, Khlong Luang, Thailand.
J Biomed Mater Res B Appl Biomater. 2023 Jun;111(6):1207-1223. doi: 10.1002/jbm.b.35226. Epub 2023 Jan 31.
Cuttlebone (CB) is a marine waste-derived biomaterial and a rich source of calcium carbonate for the biosynthesis of the calcium phosphate (CaP) particles. The current study aimed to synthesize CB derived biphasic calcium phosphate (CB-BCP) and investigate biological activity of the CB-CaP: hydroxyapatite (CB-HA), beta-tricalcium phosphate (CB-b-TCP) and biphasic 60:40 (w/w) HA/b-TCP (CB-BCP) with the human dental pulp stem cells (hDPSCs). The particles were synthesized using solid state reactions under mild condition and properties of the particles were compared with a commercial BCP as a reference material. Morphology, particle size, physicochemical properties, mineral contents, and the ion released patterns of the particles were examined. Then the particle/cell interaction, cell cytotoxicity and osteogenic property of the particles were investigated in the direct and indirect cell culture models. It was found that an average particles size of the CB-HA was 304.73 ± 4.19 nm, CB-b-TCP, 503.17 ± 23.06 nm and CB-BCP, 1394.67 ± 168.19 nm. The physicochemical characteristics of the CB-CaP were consistent with the HA, b-TCP and BCP. The highest level of calcium (Ca) was found in the mineral contents and the preincubated medium of the CB-BCP and traces of fluoride, magnesium, strontium, and zinc were identified in the CB-CaP. The cell cytotoxicity and osteogenic property of the particles were dose dependent. The particles adhered on cell surface and were internalized into the cell cytoplasm. The CB-BCP and CB-HA indirectly and directly promote osteoblastic differentiations of the hDPSCs in stronger levels than other groups. The CB-BCP and CB-HA were potential bioactive bone substitute materials.
乌贼骨（CB）是一种源自海洋废弃物的生物材料，是用于生物合成磷酸钙（CaP）颗粒的丰富碳酸钙来源。当前研究旨在合成源自乌贼骨的双相磷酸钙（CB-BCP），并研究CB-CaP（羟基磷灰石（CB-HA）、β-磷酸三钙（CB-β-TCP）以及60:40（w/w）HA/β-TCP双相物（CB-BCP））与人牙髓干细胞（hDPSCs）的生物活性。这些颗粒是在温和条件下通过固态反应合成的，并将颗粒的性质与作为参考材料的市售BCP进行了比较。研究了颗粒的形态、粒径、物理化学性质、矿物质含量以及离子释放模式。然后在直接和间接细胞培养模型中研究了颗粒与细胞的相互作用、细胞毒性和成骨特性。结果发现，CB-HA的平均粒径为304.73±4.19nm，CB-β-TCP为503.17±23.06nm，CB-BCP为1394.67±168.19nm。CB-CaP的物理化学特性与HA、β-TCP和BCP一致。在CB-BCP的矿物质含量和预孵育培养基中发现了最高水平的钙（Ca），并且在CB-CaP中鉴定出了痕量的氟、镁、锶和锌。颗粒的细胞毒性和成骨特性呈剂量依赖性。颗粒附着在细胞表面并被内化到细胞质中。CB-BCP和CB-HA间接和直接促进hDPSCs成骨分化的程度比其他组更强。CB-BCP和CB-HA是潜在的生物活性骨替代材料。
