H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, PR China.
Drug Deliv. 2023 Dec;30(1):2159587. doi: 10.1080/10717544.2022.2159587.
Multi-drug resistant (MDR) bactearial strains have posed serious health issues, thus leading to a significant increase in mortality, morbidity, and the expensive treatment of infections. Metal-organic frameworks (MOFs), comprising metal ions and a variety of organic ligands, have been employed as an effective drug deliveryy vehicle due to their low toxicity, biodegradability, higher structural integrity and diverse surface functionalities. Polydopamine (PDA) is a versatile biocompatible polymer with several interesting properties, including the ability to adhere to biological surfaces. As a result, modifying drug delivery vehicles with PDA has the potential to improve their antimicrobial properties. This work describes the preparation of PDA-coated Zn-MOFs for improving curcumin's antibacterial properties against S. aureus and E. coli. Powder X-ray diffraction (P-XRD), FT-IR, scanning electron microscopy (SEM), and DLS were utilized to characterize PDA-coated Zn-MOFs. The curcumin loading and in vitro release of the prepared MOFs were also examined. Finally, the MOFs were tested for bactericidal ability against E. coli and S. aureus using an anti-bacterial assay and surface morphological analysis. Smaller size MOFs were capable of loading and releasing curcumin. The findings showed that as curcumin was encapsulated into PDA-coated MOFs, its bactericidal potential was significantly enhanced, and the findings were further supported by SEM which indicated the complete morphological distortion of the bacteria after treatment with PDA-Cur-Zn-MOFs. These studies clearly indicate that the PDA-Cur-Zn-MOFs developed in this study are extremely promising for long-term release of drugs to treat a wide range of microbial infections.
多药耐药（MDR）细菌菌株对健康构成了严重威胁，导致死亡率、发病率上升，以及感染治疗费用昂贵。金属有机骨架（MOFs）由金属离子和各种有机配体组成，由于其低毒性、生物可降解性、更高的结构完整性和多样化的表面功能，已被用作有效的药物输送载体。聚多巴胺（PDA）是一种多功能的生物相容性聚合物，具有多种有趣的特性，包括能够附着在生物表面上。因此，用 PDA 修饰药物输送载体有可能改善其抗菌性能。这项工作描述了制备 PDA 包覆的 Zn-MOFs，以提高姜黄素对金黄色葡萄球菌和大肠杆菌的抗菌性能。利用粉末 X 射线衍射（P-XRD）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和动态光散射（DLS）对 PDA 包覆的 Zn-MOFs 进行了表征。还对制备的 MOFs 的姜黄素负载和体外释放进行了研究。最后，通过抗菌试验和表面形态分析，测试了 MOFs 对大肠杆菌和金黄色葡萄球菌的杀菌能力。较小尺寸的 MOFs 能够负载和释放姜黄素。研究结果表明，当姜黄素被包封在 PDA 包覆的 MOFs 中时，其杀菌潜力显著增强，SEM 进一步证实了这一点，SEM 表明用 PDA-Cur-Zn-MOFs 处理后，细菌的形态完全变形。这些研究清楚地表明，本研究中开发的 PDA-Cur-Zn-MOFs 非常有希望用于长期释放药物来治疗广泛的微生物感染。
