Ding Xiang, Qian Fang, Mu Guangqing, Tuo Yanfeng
School of Food Science and Technology, Dalian Polytechnic University, Dalian, P. R. China.
Dalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian, P. R. China.
Prep Biochem Biotechnol. 2023 Oct;53(9):1058-1066. doi: 10.1080/10826068.2023.2166957. Epub 2023 Jan 31.
The biomass of strains depends on the culture media and culture conditions. The purpose of this study was to optimize the culture medium composition and culture conditions of Y44 to improve its biomass. The utilization of different carbon sources and nitrogen sources by Y44 was assessed by single factor experiment to screen out the economical carbon and nitrogen sources for Y44 growth. Through optimization experiments, the optimized culture medium for Y44 growth consists of soybean peptone 44.1 g/L, yeast extract 22.1 g/L, sucrose 35.6 g/L, hydrogen diamine citrate 2 g/L, anhydrous sodium acetate 8.5 g/L, dipotassium hydrogen phosphate 4 g/L, Tween-80 2 mL/L, manganese sulfate 0.25 g/L, and magnesium sulfate 0.58 g/L, and the initial pH 6.7. The concentration of viable bacteria cells of Y44 culturing in the optimized medium at 37 °C for 16 h was up to 3.363 × 10 CFU/mL, as 6.11 times higher than that in the MRS medium.
菌株的生物量取决于培养基和培养条件。本研究的目的是优化Y44的培养基组成和培养条件以提高其生物量。通过单因素实验评估Y44对不同碳源和氮源的利用情况，以筛选出有利于Y44生长的经济碳源和氮源。通过优化实验，Y44生长的优化培养基由44.1 g/L大豆蛋白胨、22.1 g/L酵母提取物、35.6 g/L蔗糖、2 g/L柠檬酸氢二铵、8.5 g/L无水乙酸钠、4 g/L磷酸氢二钾、2 mL/L吐温-80、0.25 g/L硫酸锰和0.58 g/L硫酸镁组成，初始pH值为6.7。Y44在优化培养基中于37℃培养16 h时的活菌细胞浓度高达3.363×10 CFU/mL，比在MRS培养基中的浓度高6.11倍。
