Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center for Biological Structure & Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China; email:
Annu Rev Biophys. 2023 May 9;52:339-360. doi: 10.1146/annurev-biophys-092022-100958. Epub 2023 Jan 31.
The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-ET resolution revolution. Meanwhile, significant efforts have been made to improve the identification of targets in the cellular context and the throughput of cryo-focused ion beam (FIB) milling. Together, these developments led to a surge of in situ discoveries on how enveloped viruses are assembled and how viruses interact with cells in infected hosts. In this article, we review the recent advances in cryo-ET, high-resolution insights into virus assembly, and the findings from inside infected eukaryotic and prokaryotic cells.
近年来，冷冻电子断层扫描（cryo-ET）技术的迅速发展带来了 cryo-ET 分辨率革命。与此同时，人们也在努力提高在细胞环境中目标的识别能力和冷冻聚焦离子束（FIB）铣削的通量。这些进展共同推动了对包膜病毒组装方式以及病毒在感染宿主细胞中相互作用方式的原位发现的激增。在本文中，我们回顾了 cryo-ET 的最新进展、对病毒组装的高分辨率见解，以及从感染的真核和原核细胞内部获得的发现。
