利用冷冻电镜断层成像技术揭示细菌细胞生物学。

Revealing bacterial cell biology using cryo-electron tomography.

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Electronic address: https://twitter.com/khannakanika111.

Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA; Howard Hughes Medical Institute, University of California San Diego, La Jolla, CA 92093, USA.

出版信息

Curr Opin Struct Biol. 2022 Aug;75:102419. doi: 10.1016/j.sbi.2022.102419. Epub 2022 Jul 9.

DOI:10.1016/j.sbi.2022.102419

PMID:35820259

Abstract

Visualizing macromolecules inside bacteria at a high spatial resolution has remained a challenge owing to their small size and limited resolution of optical microscopy techniques. Recent advances in cryo-electron tomography (cryo-ET) imaging methods have revealed the spatial and temporal assemblies of many macromolecules involved in different cellular processes in bacteria at a resolution of a few nanometers in their native milieu. Specifically, the application of cryo-focused ion beam (cryo-FIB) milling to thin bacterial specimens makes them amenable for high-resolution cryo-ET data collection. In this review, we highlight recent research in three emerging areas of bacterial cell biology that have benefited from the cryo-FIB-ET technology - cytoskeletal filament assembly, intracellular organelles, and multicellularity.

摘要

在高空间分辨率下可视化细菌内的大分子一直是一个挑战，这是由于它们的体积小和光学显微镜技术的分辨率有限。最近在低温电子断层成像（cryo-ET）成像方法方面的进展揭示了许多大分子在细菌中参与不同细胞过程的空间和时间组装，其分辨率在其天然环境中可达几个纳米。具体来说，低温聚焦离子束（cryo-FIB）铣削技术应用于薄的细菌标本，使它们能够进行高分辨率的低温 ET 数据采集。在这篇综述中，我们强调了低温 FIB-ET 技术在三个新兴细菌细胞生物学领域的最新研究进展，这些领域受益于该技术：细胞骨架丝组装、细胞内细胞器和多细胞性。