School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Biol Macromol. 2023 Mar 31;232:123465. doi: 10.1016/j.ijbiomac.2023.123465. Epub 2023 Jan 28.
DEAE-52 and Sephadex G-100 columns were used to isolate Enteromorpha prolifera polysaccharide (EPP), which contains α-L-Rhap-(1 → 4)-α-L-Arap-(1 → 2)-α-L-Rhap-(1 → 3)-β-D-Galp-(1 → structural fragment, along with α-L-Rhap-(1 → and →2)-α-L-Rhap-(1 → 3)-β-D-GlcpA-(1 → side bonds that connect to →3,6)-β-D-Galp-(1→. The anti-ageing and hypoglycemic activities of EPP were assessed using an ageing diabetic mice model, and the revealed that EPP could improve glucose metabolism-associated parameters and inhibit the expression of ageing associated genes, including p16, p38 MAPK, NOX-1, VEGF, and AGER, thus preventing liver damage. Moreover, gut microbiota profiling revealed that EPP significantly increased the abundances of o_Lactobacillaceae, c_Bacilli, f_Lactobacillaceae, g_Lactobacillus, and p_Firmicutes, showing that EPP has a probiotic effect on enhancing the beneficial microbiota in ageing diabetic mice. In summary, EPP might serve as a potential bioactive compound to alleviate hyperglycaemia and ageing in diabetic in mice and further clinical studies are required to verify these effects.
使用 DEAE-52 和 Sephadex G-100 柱分离浒苔多糖 (EPP)，其含有 α-L-Rhap-(1 → 4)-α-L-Arap-(1 → 2)-α-L-Rhap-(1 → 3)-β-D-Galp-(1 → 结构片段，以及 α-L-Rhap-(1 → 和 →2)-α-L-Rhap-(1 → 3)-β-D-GlcpA-(1 → 连接到 →3,6)-β-D-Galp-(1→的侧链。使用衰老糖尿病小鼠模型评估 EPP 的抗衰老和降血糖活性，结果表明 EPP 可以改善与葡萄糖代谢相关的参数，并抑制衰老相关基因的表达，包括 p16、p38 MAPK、NOX-1、VEGF 和 AGER，从而防止肝脏损伤。此外，肠道微生物组分析表明，EPP 显著增加了 o_Lactobacillaceae、c_Bacilli、f_Lactobacillaceae、g_Lactobacillus 和 p_Firmicutes 的丰度，表明 EPP 对增强衰老糖尿病小鼠有益微生物群具有益生菌作用。总之，EPP 可能作为一种潜在的生物活性化合物，用于缓解糖尿病小鼠的高血糖和衰老，需要进一步的临床研究来验证这些效果。
