RHOA、CSNK1A1、DVL2、FZD8 和 LRP5 基因的过表达可增强幽门螺杆菌存在时胃癌的发展。

Overexpressions of RHOA, CSNK1A1, DVL2, FZD8, and LRP5 genes enhance gastric cancer development in the presence of Helicobacter pylori.

机构信息

Department of Internal Medicine, Medical Faculty, Manisa Celal Bayar University, Manisa Turkey.

Department of Medical Genetics, Medical Faculty, Aydın Adnan Menderes University, Aydın, Turkey.

出版信息

Arab J Gastroenterol. 2023 May;24(2):91-97. doi: 10.1016/j.ajg.2023.01.004. Epub 2023 Jan 29.

DOI:10.1016/j.ajg.2023.01.004

PMID:36720664

Abstract

BACKGROUND AND STUDY AIMS

Intestinal metaplasia (IM), and Helicobacter pylori (HP) infection can be shown as risk factors in the development of gastric cancer (GC). WNT signaling pathway plays a critical role in carcinogenesis. However, the literature studies are limited on the significance of this pathway for the transition from IM to GC.

PATIENTS AND METHODS

We aimed to investigate the importance of the genes of WNT signaling pathways diagnostic and prognostic markers in the presence and absence of HP in conversion from IM to GC. 104 patients, (GC group n = 35, IM group n = 45, control group n = 25) were included in this case-control study. Expression of genes in WNT signalling were searched in study groups with qRT-PCR array and qRT-PCR method. Data were analysed using PCR array data analysis software.

RESULTS

Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was detected in the GC and IM groups compared to the control group (p < 0.05). Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was observed in patients with metastatic GC compared to patients with GC without metastasis (p < 0.05). It was found that the RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes were statistically significantly over-expressed in diffuse GC patients compared to non-diffuse GC patients (p < 0.05). Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was detected in HP positive IM patients compared to HP negative IM patients (p < 0.05).

CONCLUSION

Overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes in IM may suggest that these genes are important markers in the development of IM and inflammation with HP. In addition, these genes are linked to tumor burden in the GC group. Consequently, we can conclude that these genes are poor prognosis biomarkers for GC and have the potential to be used as markers for future treatment monitoring.

摘要

背景与研究目的

肠上皮化生（IM）和幽门螺杆菌（HP）感染可被视为胃癌（GC）发展的危险因素。WNT 信号通路在癌变过程中起着关键作用。然而，关于该通路在 IM 向 GC 转化中的作用，文献研究有限。

患者和方法

我们旨在研究 WNT 信号通路基因的诊断和预后标志物在 HP 存在和不存在的情况下对 IM 向 GC 转化的重要性。本病例对照研究纳入了 104 名患者（GC 组 n=35、IM 组 n=45、对照组 n=25）。采用 qRT-PCR 芯片和 qRT-PCR 方法在研究组中搜索 WNT 信号转导基因的表达。使用 PCR 芯片数据分析软件对数据进行分析。

结果

与对照组相比，GC 组和 IM 组中 RHOA、CSNK1A1、DVL2、FZD8 和 LRP5 基因的表达显著上调（p<0.05）。与无转移 GC 患者相比，转移性 GC 患者中 RHOA、CSNK1A1、DVL2、FZD8 和 LRP5 基因的表达显著上调（p<0.05）。与非弥漫性 GC 患者相比，弥漫性 GC 患者中 RHOA、CSNK1A1、DVL2、FZD8 和 LRP5 基因的表达显著上调（p<0.05）。与 HP 阴性 IM 患者相比，HP 阳性 IM 患者中 RHOA、CSNK1A1、DVL2、FZD8 和 LRP5 基因的表达显著上调（p<0.05）。