Zhang Daiqin, Li Wenting, Hu Xin, Huang Hongyu, Zhang Xiaobing
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
Infect Drug Resist. 2023 Jan 25;16:487-497. doi: 10.2147/IDR.S393347. eCollection 2023.
The purpose of this study is to examine the variations between extra-intestinal and intestinal infections of in terms of strain types, risk factors, drug susceptibility results, and the distribution of drug resistance and virulence genes.
A total of 188 strains were identified to the species level using housekeeping genes (, and ). The risk factors for extra-intestinal and intestinal infection, as well as mortality, were retrospectively examined in this study. The broth microdilution method was used to investigate the antimicrobial susceptibility profiles. Touchdown polymerase chain reaction (PCR) assays and DNA sequencing were employed to confirm virulence and the presence of drug resistance genes.
The housekeeping genes identified 188 strains into 7 species. Extra-intestinal isolates generally contained and , while intestinal were (p=0.0001). Extra-intestinal infections (158/188) were the main type and accounted for 24/27 of all fatalities. Malignant tumors, hepatobiliary diseases, anemia, and hypoproteinemia were linked to infections. Poor results were associated with septic shock. Using the broth microdilution method, over 80% isolates were susceptible to most antimicrobials, except for ceftazidime (79.8%) and ceftriaxone (69.7%). Except for imipenem, intestinal strains were more susceptible to other medications than extra-intestinal. Using touch-down polymerase chain reaction testing and DNA sequencing, 6 strains, 31 strains, and a strain only had , , and , respectively. Two each possessed + , and + + + ; two each possessed + + + , and + . Thirty-four of the 42 strains mentioned above were isolated from extra-intestinal. , and , were in intestinal more frequently, but , and were in extra-intestinal more frequently.
inside and outside intestinal differed in their clinical characteristics, drug susceptibility, drug resistance and virulence genes.
本研究旨在从菌株类型、危险因素、药敏结果以及耐药和毒力基因分布方面，探讨肠外感染与肠道感染之间的差异。
使用管家基因（、和）将总共188株菌株鉴定到种水平。本研究回顾性分析了肠外和肠道感染的危险因素以及死亡率。采用肉汤微量稀释法研究抗菌药物敏感性谱。采用降落聚合酶链反应（PCR）检测和DNA测序来确认毒力和耐药基因的存在。
管家基因将188株菌株鉴定为7个种。肠外分离株通常含有和，而肠道分离株为（p = 0.0001）。肠外感染（158/188）是主要类型，占所有死亡病例的24/27。恶性肿瘤、肝胆疾病、贫血和低蛋白血症与感染有关。不良结果与感染性休克有关。采用肉汤微量稀释法，除头孢他啶（79.8%）和头孢曲松（69.7%）外，超过80%的分离株对大多数抗菌药物敏感。除亚胺培南外，肠道菌株比肠外菌株对其他药物更敏感。通过降落聚合酶链反应检测和DNA测序，6株菌株、31株菌株和1株菌株分别仅含有、和。2株菌株各含有 + ，以及 + + + ；2株菌株各含有 + + + + ，以及 + 。上述42株菌株中有34株从肠外分离得到。、和在肠道中出现的频率更高，但、和在肠外出现的频率更高。
肠道内外在临床特征、药敏性、耐药和毒力基因方面存在差异。
