Zhang Tingyan, Lin Yanfeng, Li Peihan, Li Zhonghong, Liu Xiong, Li Jinhui, Li Lizhong, Wang Kaiying, Liu Zhongdong, Li Peng, Lu Lanfen, Wang Hongyan
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, People's Republic of China.
Chinese PLA Center for Disease Control and Prevention, Beijing, People's Republic of China.
Infect Drug Resist. 2021 Mar 9;14:947-952. doi: 10.2147/IDR.S301736. eCollection 2021.
The emergence of multidrug-resistant poses daunting challenges to the treatment of clinical infections. The purpose of this study was to characterize the genome of a strain with an IncX3 plasmid encoding both the and genes.
Strain ZT01-0079 was isolated from a clinical urine sample. The Vitek2 system was used for identification and antimicrobial susceptibility testing. The presence of was detected by PCR and sequencing. Conjugation experiments and Southern blotting were performed to determine the transferability of the - carrying plasmid. Nanopore and Illumina sequencing were performed to better understand the genomic characteristics of the strain.
Strain ZT01-0079 was identified as , and the coexistence of and multiple drug resistance genes was confirmed. Electrophoresis and Southern blotting showed that was located on a ~53kb IncX3 plasmid. The NDM-1-encoding plasmid was successfully transferred at a frequency of 1.68×10. Both the and genes were located on the self-transferable IncX3 plasmid.
The rapid spread of the IncX3 plasmid highlights the importance of continuous monitoring of the prevalence of NDM-1-encoding . Mutations of existing carbapenem resistance genes will bring formidable challenges to clinical treatment.
多重耐药菌的出现给临床感染治疗带来了严峻挑战。本研究的目的是对一株携带编码NDM-1和blaOXA-181基因的IncX3质粒的菌株的基因组进行特征分析。
从一份临床尿液样本中分离出菌株ZT01-0079。使用Vitek2系统进行鉴定和药敏试验。通过PCR和测序检测NDM-1的存在。进行接合实验和Southern印迹分析以确定携带NDM-1的质粒的可转移性。进行纳米孔测序和Illumina测序以更好地了解该菌株的基因组特征。
菌株ZT01-0079被鉴定为肺炎克雷伯菌，证实了NDM-1和多种耐药基因的共存。电泳和Southern印迹分析表明NDM-1位于一个约53kb的IncX3质粒上。编码NDM-1的质粒以1.68×10⁻⁴的频率成功转移。NDM-1和blaOXA-181基因均位于可自我转移的IncX3质粒上。
IncX3质粒的快速传播凸显了持续监测编码NDM-1的质粒流行情况的重要性。现有碳青霉烯类耐药基因的突变将给临床治疗带来巨大挑战。
