Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Autophagy. 2023 May;19(5):1375-1377. doi: 10.1080/15548627.2023.2175305. Epub 2023 Feb 16.
Transitions from the early to late phagophore, which occur to engulf cytoplasmic material within an autophagosome for macroautophagic/autophagic degradation, involve dynamic ultrastructural changes that are not fully understood. A recent study combined cryo-electron tomography (cryo-ET) with extensive computational analysis to get a better insight into autophagosome biogenesis in situ within yeast cells. This approach disclosed new information on the shape of autophagic structures, their contacts with surrounding organelles, membrane sources, and mechanisms of transition. Together, these results provide new directions for autophagy research, and show the potential of cryo-ET in cell biology. Cryo-ET, cryo-electron tomography; ER, endoplasmic reticulum; IMDa, intermembrane distance in the autophagosome; IMDp, intermembrane distance in the phagophore; LD, lipid droplets.
从早期到晚期的噬泡的转变，即发生在自噬体中吞噬细胞质物质以进行巨自噬/自噬降解的过程，涉及到动态的超微结构变化，但这些变化还不完全清楚。最近的一项研究将冷冻电子断层扫描（cryo-ET）与广泛的计算分析相结合，以便更好地了解酵母细胞内原位自噬体的生物发生过程。该方法揭示了自噬结构的形状、它们与周围细胞器的接触、膜源以及转变机制的新信息。这些结果为自噬研究提供了新的方向，并展示了 cryo-ET 在细胞生物学中的潜力。cryo-ET，冷冻电子断层扫描；ER，内质网；IMDa，自噬体中的膜间距离；IMDp，噬泡中的膜间距离；LD，脂滴。
