School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510699, PR China.
Anal Methods. 2023 Feb 9;15(6):843-848. doi: 10.1039/d2ay01794f.
In this work, a label-free fluorescent aptasensor based on the inner filter effect (IFE) between gold nanoparticles (AuNPs) and graphene oxide quantum dots (GOQDs) was developed for the detection of kanamycin in complex samples. AuNPs are capable of functioning as the fluorescence absorber of GOQDs because of the complementary overlap between their absorption spectra and the emission spectra of GOQDs. AuNPs can effectively quench the fluorescence of GOQDs the IFE and modulate it with their aggregation state. In the presence of kanamycin, the aptamer is released from the surface of AuNPs, leading to their salt-induced aggregation and the fluorescence recovery of GOQDs. Under the optimum conditions, the fluorescence intensity of GOQDs was linearly proportional to the concentration of kanamycin over the range from 5 to 600 nM, with a detection limit of 3.6 nM. Moreover, the fluorescent aptasensor was successfully applied for kanamycin detection in complex samples (milk, honey and serum), which might hold great promise for kanamycin detection in food safety control and clinical research.
在这项工作中，开发了一种基于金纳米粒子（AuNPs）和氧化石墨烯量子点（GOQDs）之间的内滤效应（IFE）的无标记荧光适体传感器，用于检测复杂样品中的卡那霉素。由于 AuNPs 的吸收光谱与 GOQDs 的发射光谱之间存在互补重叠，因此它们可以作为 GOQDs 的荧光吸收剂。AuNPs 可以有效地猝灭 GOQDs 的荧光 通过其聚集状态来调制 IFE。在存在卡那霉素的情况下，适体从 AuNPs 表面释放出来，导致其盐诱导聚集和 GOQDs 的荧光恢复。在最佳条件下，GOQDs 的荧光强度与卡那霉素的浓度在 5 至 600 nM 范围内呈线性比例关系，检测限为 3.6 nM。此外，该荧光适体传感器成功应用于复杂样品（牛奶、蜂蜜和血清）中的卡那霉素检测，这可能为食品安全控制和临床研究中的卡那霉素检测提供了很大的前景。
