Department of Chemistry, RCSI, 123 St Stephen's Green, Dublin 2, Ireland.
Chem Commun (Camb). 2023 Feb 14;59(14):1951-1954. doi: 10.1039/d2cc06461h.
Sequential azide/diyne cycloadditions proved highly effective for the macrocyclization of a bis-azido aza-dipyrrin. Macrocyclic aza-dipyrrin could be produced in 30 min at rt in water with changes in fluorescence intensity and lifetimes measurable upon reaction. Live cell microscopy showed that aza-dipyrrins were suitable for confocal and STED super-resolution imaging and a bioorthogonal response to macrocyclization could be detected in cellular compartments. These results will encourage a broader examination of the sensing and imaging uses of aza-dipyrrins.
连续的叠氮化物/二炔环加成反应对双叠氮化物氮杂二吡咯的大环化反应非常有效。在水中，室温下 30 分钟内即可生成大环氮杂二吡咯，反应后可测量荧光强度和寿命的变化。活细胞显微镜显示，氮杂二吡咯适用于共聚焦和 STED 超分辨率成像，并且可以在细胞区室中检测到大环化的生物正交反应。这些结果将鼓励更广泛地研究氮杂二吡咯的传感和成像用途。
