State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Analyst. 2023 Feb 27;148(5):985-994. doi: 10.1039/d2an02043b.
In 2022, the outbreak of the monkeypox virus occurred in many non-endemic countries, and the World Health Organization (WHO) assessed that this outbreak was "atypical". The establishment of a rapid and effective assay that can be used for the early diagnosis of monkeypox virus infection is crucial for outbreak prevention and control. In this study, the monkeypox virus A29 protein and the homologous vaccinia virus A27 protein and cowpox virus 162 protein were expressed in for screening. We synthesized the monkeypox virus A29 peptide as the immunogen and obtained 25 monoclonal antibodies (mAbs) against the A29 protein using mouse hybridoma techniques. Then an immunochromatographic test strip method for detecting A29 was established. The strips utilizing mAb-7C5 and 5D8 showed the best sensitivity and lowest limit of detection: 50 pg mL for purified A29 and specificity tests showed that the strips did not cross-react with other orthopox viruses (vaccinia virus or cowpox virus) as well as common respiratory pathogens (SARS-CoV-2, influenza A and influenza B). Therefore, this method can be used for early and rapid diagnosis of monkeypox virus infection by antigen detection.
2022 年，猴痘病毒在多个非流行国家暴发，世界卫生组织（WHO）评估此次疫情为“非典型”。建立一种快速有效的检测方法，用于猴痘病毒感染的早期诊断，对于疫情的防控至关重要。本研究在 中表达了猴痘病毒 A29 蛋白及其同源的牛痘病毒 A27 蛋白和正痘病毒 162 蛋白，用于筛选。我们合成了猴痘病毒 A29 肽作为免疫原，利用小鼠杂交瘤技术获得了 25 株针对 A29 蛋白的单克隆抗体（mAbs）。然后建立了一种用于检测 A29 的免疫层析测试条方法。利用 mAb-7C5 和 5D8 的条带显示出最佳的灵敏度和最低检测限：纯化 A29 的 50pg/mL，特异性试验表明，该条带与其他正痘病毒（牛痘病毒或正痘病毒）以及常见呼吸道病原体（SARS-CoV-2、甲型流感和乙型流感）没有交叉反应。因此，该方法可用于通过抗原检测对猴痘病毒感染进行早期和快速诊断。
