Silva Luiz Ricardo G, Stefano Jéssica S, Kalinke Cristiane, Crapnell Robert D, Brazaca Laís C, Marcolino-Junior Luiz H, Bergamini Marcio F, Banks Craig E, Janegitz Bruno C
Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil.
Institute of Chemistry, University of Campinas (Unicamp), São Paulo 13083-859, Brazil.
ACS Omega. 2024 Jul 17;9(30):33099-33110. doi: 10.1021/acsomega.4c04460. eCollection 2024 Jul 30.
Herein, we present the first 3D-printed electrochemical portable biodevice for the detection of monkeypox virus (MKPV). The electrochemical device consists of two biosensors: an immunosensor and a genosensor specifically designed for the detection of the protein A29 and a target DNA of MKPV, respectively. The electrodes were manufactured using lab-made ultraflexible conductive filaments composed of carbon black, recycled PLA from coffee pods, and castor oil as a plasticizer. The sensors created through 3D printing technology exhibited good reproducibility and repeatability of analytical responses. Furthermore, both the immunosensor and genosensor demonstrated excellent MKPV detection capabilities, with a linear range from 0.01 to 1.0 μmol L for the antigen and 0.1 to 20.0 μmol L for the DNA target. The biosensors achieved limits of detection of 2.7 and 29 nmol L for the immunosensor and genosensor, respectively. Interference tests conducted with the biosensors demonstrated their selectivity for MKPV. Moreover, analyses of fortified human serum samples showed recoveries close to 100%, confirming the absence of significant matrix effects for MKPV analysis. Therefore, the 3D-printed multiplex device represents a viable and highly promising alternative for on-site, portable, and rapid point-of-care MKPV monitoring.
在此，我们展示了首个用于检测猴痘病毒（MKPV）的3D打印电化学便携式生物装置。该电化学装置由两个生物传感器组成：一个免疫传感器和一个基因传感器，分别专门设计用于检测MKPV的A29蛋白和靶DNA。电极是使用实验室自制的超柔性导电细丝制造的，该细丝由炭黑、咖啡胶囊回收的聚乳酸（PLA）和作为增塑剂的蓖麻油组成。通过3D打印技术制造的传感器在分析响应方面表现出良好的重现性和重复性。此外，免疫传感器和基因传感器都表现出出色的MKPV检测能力，抗原的线性范围为0.01至1.0 μmol/L，DNA靶标的线性范围为0.1至20.0 μmol/L。免疫传感器和基因传感器的检测限分别为2.7和29 nmol/L。对生物传感器进行的干扰测试证明了它们对MKPV的选择性。此外，对加标人血清样本的分析显示回收率接近100%，证实了MKPV分析中不存在显著的基质效应。因此，这种3D打印的多重检测装置是现场、便携式和快速即时护理MKPV监测的一种可行且极有前景的替代方案。
