天然产物生物合成中的酶促十氢萘形成。
Enzymatic Decalin Formation in Natural Product Biosynthesis.
机构信息
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.
CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
出版信息
J Am Chem Soc. 2023 Feb 15;145(6):3301-3305. doi: 10.1021/jacs.2c12854. Epub 2023 Feb 1.
Abstract
Stereoselective synthesis of decalin structures using [4 + 2] cycloaddition is challenging. We explored the biosynthetic pathway of the fungal natural product fischerin () to identify a new pericyclase FinI that can catalyze such a reaction. The cocrystal structure of FinI, a predicted methyltransferase, with the product and SAM provides insight into decalin formation in nature.
摘要
使用[4 + 2]环加成反应进行十氢萘结构的立体选择性合成具有挑战性。我们探索了真菌天然产物 Fischerin（）的生物合成途径，以鉴定能够催化此类反应的新过氧化物酶 FinI。FinI 的晶体结构（一种预测的甲基转移酶）与产物和 SAM 的共晶结构提供了对自然界中十氢萘形成的深入了解。
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