Zhu Yulian, Guo Xiaomei, Ma Xiao, Liu Kai, Han Yuting, Wu Yongquan, Li Xun
School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou 341000, P. R. China.
Analyst. 2023 Feb 27;148(5):1016-1023. doi: 10.1039/d2an01994a.
Abnormal levels of thiols in cysteine (Cys) have been shown to be associated with growth retardation, skin lesions, and neurotoxicity in humans. Herein, we designed and synthesized a rare earth upconversion luminescent (UCL) nanocomposite probe UCNP-PEG-NOF1 for the UCL detection of Cys using NOF1 developed by our group as a Cys probe. The core structure of rare earth nanoparticles can absorb light at 980 nm and convert it into visible light. The detection principle of Cys was based on the change in absorption peak before and after the reaction between NOF1 and Cys, as well as the change in UCL intensity. The rare earth nanocomposite in the probe could be excited by near-infrared light and had low background fluorescence and strong penetration ability; thus, the probe was successfully employed to specifically and sensitively detect Cys with a low background signal. Overall, the developed UCNP-PEG-NOF1 probe had good selectivity and high sensitivity for Cys; its detection limit was as low as 83 nM.
已证明，人体内半胱氨酸（Cys）中硫醇水平异常与生长发育迟缓、皮肤损伤及神经毒性有关。在此，我们设计并合成了一种稀土上转换发光（UCL）纳米复合探针UCNP-PEG-NOF1，用于利用本课题组开发的NOF1作为Cys探针，通过UCL检测Cys。稀土纳米颗粒的核心结构可吸收980nm的光并将其转换为可见光。Cys的检测原理基于NOF1与Cys反应前后吸收峰的变化以及UCL强度的变化。探针中的稀土纳米复合材料可被近红外光激发，背景荧光低且穿透能力强；因此，该探针成功用于以低背景信号特异性、灵敏地检测Cys。总体而言，所开发的UCNP-PEG-NOF1探针对Cys具有良好的选择性和高灵敏度；其检测限低至83 nM。
