Department of Chemistry, Changwon National University, Changwon, 51140, Republic of Korea.
Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
Anal Bioanal Chem. 2021 Feb;413(5):1363-1371. doi: 10.1007/s00216-020-03099-1. Epub 2021 Jan 3.
Novel polyepinephrine-modified NaYF:Yb,Tm upconversion luminescent nanoparticles (UCNP@PEP) were prepared via the self-polymerization of epinephrine on the surfaces of the UCNPs for selective sensing of Fe inside a cell and for intracellular imaging. The proposed UCNP@PEP probe is a strong blue light emitter (λ = 474 nm) upon exposure to an excitation wavelength of 980 nm. The probe was used for detecting Fe owing to the complexation reaction between UCNP@PEP and Fe, resulting in reduced upconversion luminescence (UCL) intensity. The proposed probe has a detection limit of 0.2 μM and a good linear range of 1-10 μM for sensing Fe ions. Moreover, the UCNP@PEP probe displays high cell viability (90%) and is feasible for intracellular imaging. The ability of the probe to sense Fe in a human serum sample was tested and shows promising output for diagnostic purposes. The prepared UCNP@PEP probe was characterized by using UV-visible (UV-Vis) absorption spectrometry, fluorescence (FL) spectrometry, field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR).
新型聚肾上腺素修饰的 NaYF:Yb,Tm 上转换发光纳米粒子（UCNP@PEP）通过肾上腺素在 UCNP 表面的自聚合制备，用于细胞内铁的选择性传感和细胞内成像。在暴露于 980nm 的激发波长下，所提出的 UCNP@PEP 探针是一个强蓝光发射器（λ=474nm）。探针由于 UCNP@PEP 与 Fe 之间的络合反应，用于检测 Fe，导致上转换发光（UCL）强度降低。该探针对 Fe 离子的检测限为 0.2μM，线性范围为 1-10μM。此外，UCNP@PEP 探针具有高细胞活力（90%），可用于细胞内成像。测试了探针在人血清样本中检测 Fe 的能力，显示出有希望用于诊断目的的结果。通过使用紫外-可见（UV-Vis）吸收光谱、荧光（FL）光谱、场发射扫描电子显微镜（FE-SEM）、X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FT-IR）对制备的 UCNP@PEP 探针进行了表征。
