Li Hua-Li, Liang Jie-Ling, Li Shun-Xing, Liu Feng-Jiao, Luo Jia-Yi, Huang Zhao-Jing, Zheng Feng-Ying, Su Ling-Ling, Zhuo You-Bin
College of Chemistry, Chemical Engineering & Environmental Science, Minnan Normal University, Zhangzhou, 363000, China.
Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Fujian Province University Key Laboratory of Pollution Monitoring and Control, Minnan Normal University, Zhangzhou, 3630003, China.
Mikrochim Acta. 2025 Jan 28;192(2):108. doi: 10.1007/s00604-025-06962-z.
The detection of cysteine (Cys) and homocysteine (Hcy) in biological fluids has great significance for early diagnosis, including Alzheimer's and Parkinson's disease. The simultaneous determination of Cys and Hcy with a single probe is still a huge challenge. To enlarge the differences in space structure (line and ring) and energy (-721.78 and -761.08 Hartree) between Cys and Hcy, and to cause a difference of aggregation kinetics, gold nanoparticles (AuNPs) are capped with hydrophilic and low-toxic polyvinylpyrrolidone (PVP) (named AuNPs@PVP) and some surface-active sites of AuNPs are masked, the active area for the binding between AuNPs and the detection object is confined, meanwhile, the stability of AuNPs is improved. A novel nanosensor based on confined active area and aggregation kinetics of AuNPs@PVP, is designed for the identification and determination of Cys and Hcy in 1 and 3 min, respectively, with sufficiently low detection limit (4.12 and 4.35 μM) and linear range (4.12-100 μM) for health evaluation. This single colorimetric sensor was applied successfully to the determination of urine and serum, evidencing high anti-interference ability.
生物体液中半胱氨酸（Cys）和同型半胱氨酸（Hcy）的检测对于包括阿尔茨海默病和帕金森病在内的早期诊断具有重要意义。使用单一探针同时测定Cys和Hcy仍然是一个巨大的挑战。为了扩大Cys和Hcy在空间结构（线性和环状）和能量（-721.78和-761.08哈特里）上的差异，并引起聚集动力学的差异，用亲水性和低毒性的聚乙烯吡咯烷酮（PVP）包覆金纳米颗粒（AuNPs）（命名为AuNPs@PVP），掩盖AuNPs的一些表面活性位点，限制AuNPs与检测对象结合的活性区域，同时提高AuNPs的稳定性。设计了一种基于AuNPs@PVP受限活性区域和聚集动力学的新型纳米传感器，分别在1分钟和3分钟内用于识别和测定Cys和Hcy，具有足够低的检测限（4.12和4.35μM）和线性范围（4.12 - 100μM）用于健康评估。这种单一的比色传感器已成功应用于尿液和血清的测定，证明具有高抗干扰能力。
