Melnychuk Iryna, Lizogub Viktor G
O.O. BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE.
Wiad Lek. 2022;75(12):2994-2999. doi: 10.36740/WLek202212117.
The aim: To check changes of gut microbiota composition and its metabolites in atherosclerosis (AS) patients with or without atrial fibrillation (AF) and special connections between them and important clinic and laboratory features of investigated groups.
Materials and methods: 300 patients were investigated. All investigated were divided into 3 groups: control group (CG) - 27 patients without AS and arrhythmias; mean group - 149 patients with AS but without arrhythmias; comparable group - 124 patients with AS and AF paroxysm. By 16-S rRNA sequencing was checked gut microbiota composition. The level of trimethylamine-N-oxide (TMAO), trimethylamine (TMA) plasma was determined by gas chromatography with mass electron detection.
Results: The mean and comparable groups have the significant abundance of total bacterial mass, Bacteroides Spp., Faecalibacterium Prausnitzii, Actinobacter Spp. and decreas¬ing Ruminococcus Spp. In the comparable group to the mean significant increasing of Actinobacter Spp. and decreasing Eubacterium Rectale, Ruminococcus Spp. were checked. Bacteroides Fragilis Group/ Faecalibacterium Prausnitzii ratio was significantly higher than in patients' comparable group. In the mean group patients compared with CG significant abundance of Streptococcus Spp. was checked. In the comparable group compared with CG significant leak of Eubacterium Rectale was checked. The highest amount of correlations was between Lactobacillus Spp., Streptococcus Spp. and clinic-laboratory changes. The mean and comparable groups the significant increasing of TMA ta TMAO plasma levels were checked. In patients of comparable group compared with patients mean group the significant increasing of TMAO plasma level was revealed.
Conclusions: We checked special bacterial changes of gut microbiota that are common for patients with AS and AF comparable with AS patients. TMAO plasma levels are increased significantly for patients with AS and AF comparable with AS patients. Connections between AS and AF with TMAO plasma levels are confirmed by reliable correlations between TMAO and age, BMI, GFR, HDL levels. Special bacterial species are closely connected with age, BMI, GFR, HDL, LDL, plasma TMA and TMAO levels.
本研究旨在检测有无心房颤动（AF）的动脉粥样硬化（AS）患者肠道微生物群组成及其代谢产物的变化，以及它们与研究组重要临床和实验室特征之间的特殊联系。
研究对象为300例患者。所有研究对象分为3组：对照组（CG）——27例无AS和心律失常的患者；平均组——149例有AS但无心律失常的患者；可比组——124例有AS且有AF发作的患者。通过16-S rRNA测序检测肠道微生物群组成。采用气相色谱-质谱联用仪测定血浆三甲胺-N-氧化物（TMAO）、三甲胺（TMA）水平。
平均组和可比组的总细菌量、拟杆菌属、普拉梭菌、放线菌属丰度显著增加，而瘤胃球菌属减少。与平均组相比，可比组中放线菌属显著增加，直肠真杆菌、瘤胃球菌属减少。脆弱拟杆菌群/普拉梭菌比值显著高于可比组患者。与CG组相比，平均组患者中链球菌属丰度显著增加。与CG组相比，可比组中直肠真杆菌显著减少。乳酸菌属、链球菌属与临床实验室变化之间的相关性最高。平均组和可比组血浆TMA和TMAO水平显著升高。与平均组患者相比，可比组患者血浆TMAO水平显著升高。
我们检测到AS合并AF患者与AS患者常见的肠道微生物群特殊细菌变化。与AS患者相比，AS合并AF患者血浆TMAO水平显著升高。TMAO与年龄、BMI、GFR、HDL水平之间的可靠相关性证实了AS和AF与血浆TMAO水平之间的联系。特殊细菌种类与年龄、BMI、GFR、HDL、LDL、血浆TMA和TMAO水平密切相关。
