State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, China.
Chem Commun (Camb). 2023 Feb 16;59(15):2056-2071. doi: 10.1039/d2cc06556h.
Biomarkers are essential in biology, physiology, and pharmacology; thus, their detection is of extensive importance. Fluorescent probes provide effective tools for detecting biomarkers exactly. Excited state intramolecular proton transfer (ESIPT), one of the significant photophysical processes that possesses specific photoisomerization between Keto and Enol forms, can effectively avoid annoying interference from the background with a large Stokes shift. Hence, ESIPT is an excellent choice for biomarker monitoring. Based on the ESIPT process, abundant probes were designed and synthesized using three major design methods. In this review, we conclude probes for 14 kinds of biomarkers based on ESIPT explored in the past five years, summarize these general design methods, and highlight their application for biomarker detection or .
生物标志物在生物学、生理学和药理学中至关重要；因此，对其进行检测具有广泛的重要性。荧光探针为准确检测生物标志物提供了有效的工具。激发态分子内质子转移（ESIPT）是一种重要的光物理过程，它在酮式和烯醇式之间具有特定的光异构化，可以有效地避免大斯托克斯位移带来的背景干扰。因此，ESIPT 是生物标志物监测的绝佳选择。基于 ESIPT 过程，使用三种主要的设计方法设计并合成了丰富的探针。在这篇综述中，我们总结了过去五年中基于 ESIPT 探索的 14 种生物标志物的探针，总结了这些一般的设计方法，并强调了它们在生物标志物检测中的应用。
