Roy Subhra Kanti, Moser Sandra, Dürr-Mayer Tobias, Hinkelmann Rahel, Jessen Henning J
Institute of Organic Chemistry, Albert-Ludwigs-Universität Freiburg, Albertstraße 21, 79104 Freiburg im Breisgau, Germany.
CIBSS-Centre for Integrative Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany.
Org Biomol Chem. 2025 Feb 5;23(6):1373-1379. doi: 10.1039/d4ob01926a.
We introduce two water-soluble excited state intramolecular proton transfer (ESIPT) based fluorescent turn-on probes responding to inorganic polyphosphates. These ESIPT probes enable specific detection of short-chain inorganic polyphosphates over a range of different condensed phosphates. The probes are weakly emissive in their off-state due to the blocking of ESIPT by Cu coordination. Removal of the copper ion through decomplexation by the analyte accesses the on-state. The probes detect polyphosphates over other biologically occurring phosphates, pyrophosphate, and nucleotides such as ATP, ADP, GTP. An optimal fluorescence response is observed with the short-chain polyphosphate polyP. Furthermore, the probe shows selectivity towards linear polyphosphates over cyclic metaphosphates. The rapid 'turn-off-turn-on' fluorescence responses upon consecutive addition of Cu and polyP are reversible, further highlighting sensor performance in an aqueous environment. One of the sensors is then used to monitor polyP digestion by an exopolyphosphatase (PPX).
我们介绍了两种基于水溶性激发态分子内质子转移（ESIPT）的荧光开启探针，用于检测无机多磷酸盐。这些ESIPT探针能够在一系列不同的缩合磷酸盐中特异性检测短链无机多磷酸盐。由于铜配位对ESIPT的阻断，探针在关闭状态下发射较弱。通过分析物的去络合作用去除铜离子可进入开启状态。这些探针能够检测多磷酸盐，而不受其他生物体内存在的磷酸盐、焦磷酸盐和核苷酸（如ATP、ADP、GTP）的干扰。短链多磷酸盐polyP能产生最佳的荧光响应。此外，该探针对线性多磷酸盐的选择性高于环状偏磷酸盐。连续添加铜和多磷酸盐后快速的“关闭-开启”荧光响应是可逆的，进一步突出了该传感器在水性环境中的性能。然后，其中一种传感器被用于监测外切多磷酸酶（PPX）对多磷酸盐的消化作用。
