Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Lab Chip. 2023 Feb 14;23(4):785-792. doi: 10.1039/d2lc01153k.
Automated manipulation of discrete droplets by digital microfluidics (DMF) combined with chemiluminescence (CL) is promising to achieve automated and sensitive biosensing and bioanalysis. Herein, a DMF-CL device was built to automatically study CL kinetics and biosensing of a glow-type CL reaction. Copper-cysteine nanoparticles (Cu/CysNP) were synthesized as a new CL catalyst to extend the CL reaction of luminol-HO to more than 10 min. The automated manipulation of droplets reduced reagent costs and manual errors, leading to real-time, automated, and reliable study of CL kinetics. The CL kinetics curve collected by the DMF-CL integration device is in accordance with that of a commercial CL analyser. The long-lasting luminescence ensured automated, sensitive, and reliable detection of HO as a direct or indirect analyte of the cascade catalytic reaction. Moreover, an innovative asymmetrical splitting method is proposed to quickly and precisely generate daughter droplets to ensure uniformity of the droplets and good repeatability of the DMF-CL measurements. Therefore, the DMF-CL analysis holds great potential for achieving online and automatic analysis and biosensing.
通过数字微流控（DMF）与化学发光（CL）相结合对离散液滴进行自动化操控有望实现自动化、高灵敏度的生物传感和生物分析。在此，构建了一个 DMF-CL 装置，以自动研究闪光型 CL 反应的 CL 动力学和生物传感。铜-半胱氨酸纳米粒子（Cu/CysNP）被合成用作新的 CL 催化剂，将鲁米诺-HO 的 CL 反应延长至 10 分钟以上。液滴的自动化操控降低了试剂成本并减少了人工误差，从而实现了 CL 动力学的实时、自动化和可靠研究。通过 DMF-CL 集成装置采集的 CL 动力学曲线与商业 CL 分析仪一致。持久的发光确保了 HO 的自动化、灵敏和可靠检测，HO 可以作为级联催化反应的直接或间接分析物。此外，提出了一种创新的不对称分裂方法，可快速、精确地生成子液滴，从而确保液滴的均匀性和 DMF-CL 测量的良好重复性。因此，DMF-CL 分析在实现在线和自动分析及生物传感方面具有巨大潜力。
