Zhao Yong, Jiang Shuyue, Cai Gaozhe, Wang Lihua, Zhao Jianlong, Feng Shilun
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
School of Graduate Study, University of Chinese Academy of Sciences, Beijing, 100049, China.
Microsyst Nanoeng. 2025 May 12;11(1):82. doi: 10.1038/s41378-025-00914-6.
Digital microfluidic (DMF) technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets. However, most DMF systems usually rely on complex electrode fabrication and high driving voltages. Sensor integration in DMF systems is also quite rare. In this study, a programmable magnetic digital microfluidic (PMDMF) platform integrated with electrochemical detection system was proposed. It enables non-contact, flexible droplet manipulation without complex processes and high voltages, meeting the requirements of automated electrochemical detection. The platform includes a magnetic control system, a microfluidic chip, and an electrochemical detection system. The magnetic control system consists of a microcoil array circuit board, a N52 permanent magnet, and an Arduino control module. N52 magnets generate localized magnetic fields to drive droplet movement, while the Arduino module enables programmable control for precise manipulation. The maximum average velocity of the droplet is about 3.9 cm/s. The microfluidic chip was fabricated using 3D printing and the superhydrophobic surface of chip was fabricated by spray coating. The electrochemical detection system consists of the MoS@CeO/PVA working electrode, Ag/AgCl reference electrode, and carbon counter electrode. To evaluate the practical value of the integrated platform, glucose in sweat was automatically and accurately detected. The proposed platform has a wide linear detection range (0.01-0.25 mM), a lower LOD (6.5 μM), a superior sensitivity (7833.54 μA·mM·cm), and excellent recovery rate (88.1-113.5%). It has an extensive potential for future application in the fields of medical diagnostics and point-of-care testing.
数字微流控（DMF）技术因其在操控液滴方面的准确性和灵活性而被广泛应用于生物分析和化学反应。然而，大多数DMF系统通常依赖复杂的电极制造和高驱动电压。DMF系统中的传感器集成也相当罕见。在本研究中，提出了一种集成电化学检测系统的可编程磁性数字微流控（PMDMF）平台。它能够实现非接触、灵活的液滴操控，无需复杂的工艺和高电压，满足自动化电化学检测的要求。该平台包括磁控系统、微流控芯片和电化学检测系统。磁控系统由微线圈阵列电路板、N52永磁体和Arduino控制模块组成。N52磁体产生局部磁场以驱动液滴移动，而Arduino模块实现可编程控制以进行精确操控。液滴的最大平均速度约为3.9厘米/秒。微流控芯片采用3D打印制造，芯片的超疏水表面通过喷涂制造。电化学检测系统由MoS@CeO/PVA工作电极、Ag/AgCl参比电极和碳对电极组成。为了评估该集成平台的实际价值，对汗液中的葡萄糖进行了自动准确检测。所提出的平台具有宽线性检测范围（0.01 - 0.25毫摩尔）、较低的检测限（6.5微摩尔）、优异的灵敏度（7833.54微安·毫摩尔·厘米）和出色的回收率（88.1 - 113.5%）。它在医学诊断和即时检测领域具有广泛的未来应用潜力。
