Roa Cordero Martha Viviana, Romero Pineda María Fernanda, Guerrero Rodríguez Julián Mauricio, López Ortíz José Gabriel, Leal Pinto Sandra Milena
Universidad de Santander, Facultad de Ciencias Médicas y de la Salud, Grupo de investigación en biotecnología Agroambiental y salud-Microbiota, Bucarmanga, Colombia.
Universidad de Santander, Facultad de Ciencias Médicas y de la Salud, Grupo de Investigación Manejo Clínico-Cliniudes, Bucarmanga, Colombia.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2023;58(1):31-38. doi: 10.1080/10934529.2023.2172267. Epub 2023 Feb 1.
The antimicrobial activity and biological efficiency of silver nanoparticles (AgNps) have been widely described and can be modeled through stabilizing and reducing agents, especially if they exhibit biocidal properties, which can enhance bioactivity against pathogens. The selective action of AgNps remains a major concern. In this regard, the use of plant extracts for the green synthesis of nanoparticles offers advantages because it improves the toxicity of Nps for microorganisms and is harmless to normal cells. However, biological evaluations of the activity of AgNps synthesized using different reducing agents are determined independently, and comparisons are frequently overlooked. Thus, we investigated and compared the antifungal and cytotoxic effects of two ecological AgNps synthesized from aqueous leaf extract (AgNp-M) and glucose (AgNp-G) against azole-resistant clinical isolates of spp. and nontumor mammalian cells. Synthesized AgNps exhibited an antifungal effect on planktonic cells of drug-resistant and (MIC 0.21-52.6 µg/mL). The toxicity was influenced by size. However, the use of extracts allows us to obtain AgNps that are highly selective and nongenotoxic to Vero cells due to modifications of the shape and surface. Therefore, these results suggest that AgNp-M has antimicrobial potential and deserves further investigation for biomedical applications.
银纳米颗粒（AgNps）的抗菌活性和生物学效率已被广泛描述，并且可以通过稳定剂和还原剂进行模拟，特别是当它们具有杀菌特性时，这可以增强对病原体的生物活性。AgNps的选择性作用仍然是一个主要问题。在这方面，使用植物提取物进行纳米颗粒的绿色合成具有优势，因为它提高了纳米颗粒对微生物的毒性，并且对正常细胞无害。然而，使用不同还原剂合成的AgNps活性的生物学评估是独立进行的，比较常常被忽视。因此，我们研究并比较了由水叶提取物（AgNp-M）和葡萄糖（AgNp-G）合成的两种生态AgNps对耐唑类临床分离株和非肿瘤哺乳动物细胞的抗真菌和细胞毒性作用。合成的AgNps对耐药菌和的浮游细胞表现出抗真菌作用（MIC 0.21-52.6μg/mL）。毒性受尺寸影响。然而，使用提取物使我们能够获得由于形状和表面修饰而对Vero细胞具有高度选择性且无基因毒性的AgNps。因此，这些结果表明AgNp-M具有抗菌潜力，值得在生物医学应用中进一步研究。
