Pollo Favio, Salinas Zulma, Baraquet Mariana, Otero Manuel A, Grenat Pablo R, Salas Nancy, Martino Adolfo L, Sinsch Ulrich
Ecología, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional N° 36-km 601, Río Cuarto X5804BYA, Argentina.
Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente, Consejo Nacional de Investigaciones Científicas y Técnicas, Rosario S2000EZ, Argentina.
Animals (Basel). 2023 Nov 18;13(22):3566. doi: 10.3390/ani13223566.
We provide the first evidence for hemoparasites in the endemic Cordoba treefrog . We collected 37 adult frogs at 1200 m a.s.l. in the Comechingones Mountains in the Córdoba province (Argentina). Each individual was sexed, then snout-vent length and body mass were recorded, a toe was collected for skeletochronological age determination, and a slide with a blood smear was prepared for hemoparasite screening, before releasing the frogs in situ. A total of 81% (n = 30) of the frogs were infected by hemogregarines and trypanosomes with a high intensity of infections. was found for the first time in Argentina. Hemoparasites had no significant effect on the leukocyte profile, which we assessed from the May-Grünwald-Giemsa-stained blood smears. The neutrophils/lymphocytes ratio, indicative of stress, was insignificantly higher (0.06) in parasitized frogs than in parasite-free individuals (0.04). Infected frogs were larger than the controls, but this effect vanished when correcting size data for age. Young frogs (first-breeders) dominated the age distribution of parasite-free individuals, suggesting that infection of frogs takes usually place after sexual maturation. Vectors transmitting hemoparasites to remain to be identified. We demonstrate that moderate to high intensities of hemoparasites do not significantly affect the cellular immune response of , or any of the life-history traits studied, nor did they show any external sign of disease.
我们提供了关于特有物种科尔多瓦树蛙血液寄生虫的首个证据。我们在阿根廷科尔多瓦省科梅钦戈内斯山脉海拔1200米处采集了37只成年树蛙。对每只树蛙进行性别鉴定，然后记录其吻肛长度和体重，采集一个趾头用于骨骼年代测定，制备一张血涂片载玻片用于血液寄生虫筛查，之后将树蛙放归原地。共有81%（n = 30）的树蛙感染了血簇虫和锥虫，且感染强度很高。[此处原文缺失具体寄生虫名称]在阿根廷首次被发现。血液寄生虫对白细胞谱没有显著影响，我们通过May-Grünwald-Giemsa染色的血涂片对白细胞谱进行了评估。表明应激的中性粒细胞/淋巴细胞比率在受寄生虫感染的树蛙中略高（0.06），但与未感染寄生虫的个体（0.04）相比差异不显著。受感染的树蛙比对照组的树蛙体型更大，但在校正年龄后的体型数据时，这种影响消失了。未感染寄生虫个体的年龄分布以幼蛙（首次繁殖者）为主，这表明树蛙的感染通常发生在性成熟之后。将血液寄生虫传播给[此处原文缺失具体物种名称]的媒介仍有待确定。我们证明，中等至高强度的血液寄生虫不会显著影响[此处原文缺失具体物种名称]的细胞免疫反应或所研究的任何生活史特征，它们也没有表现出任何疾病的外部迹象。
