Liu Bao-Cai, Chen Jing-Ying, Zhang Wu-Jun, Huang Ying-Zhen, Zhao Yun-Qing, Ye Jing
Agricultural Bioresource Institute, Fujian Academy of Agricultural Sciences Fuzhou 350003, China Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences Fuzhou 350003, China.
Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences Fuzhou 350003, China.
Zhongguo Zhong Yao Za Zhi. 2023 Jan;48(1):45-51. doi: 10.19540/j.cnki.cjcmm.20221016101.
Violet root rot is one of the main root diseases in the production process of Pseudostellaria heterophylla. To clarify the pathogenic species that cause the violet root rot of P. heterophylla in Fujian province, the roots and the sclerotia with violet root rot symptoms were collected from the main producing areas of P. heterophylla(Fujian province) from 2017 to 2021, and the pathogens were isolated by tissue separation method and identified by morphology and multi-gene phylogenetic analysis. Additionally, the biological characteristics of the pathogens were studied and the fungicides were determined. The results showed that 78 strains of violet root rot were isolated from the collected root samples, which belonged to one type after preliminary morphological identification. Two represen-tative strains were selected from the pathogens for multi-gene phylogenetic analysis, and they were clustered with Helicobasidium mompa together. The suitable culture conditions for the mycelium were OA medium, 25 ℃, pH 6, and ammonium oxalate as the nitrogen source. The lethal temperature of the mycelium was 50 ℃ for 10 minutes. Moreover, 99.1% propiconazole and 98.7% azoxystrobin had the optimal bacteriostatic effect, and the concentrations with the 50% bacteriostatic rate were 16.85 and 12.24 μg·mL~(-1), respectively. On the basis of the above results, the pathogen causing violet root rot of P. heterophylla in Fujian province was H. mompa. The medium type, growth temperature, pH value, nitrogen source, etc. had significant effect on the growth of mycelium.
紫纹羽病是太子参生产过程中的主要根部病害之一。为明确福建省导致太子参紫纹羽病的病原菌种类，于2017年至2021年从福建省太子参主产区采集具有紫纹羽病症状的根部及菌核，采用组织分离法分离病原菌，并通过形态学和多基因系统发育分析进行鉴定。此外，对病原菌的生物学特性进行了研究并测定了杀菌剂。结果表明，从采集的根部样本中分离出78株紫纹羽病病原菌，初步形态鉴定后属于同一类型。从病原菌中选取两株代表性菌株进行多基因系统发育分析，它们与桑卷担菌聚在一起。菌丝体适宜的培养条件为燕麦片培养基、25℃、pH值6、以草酸铵为氮源。菌丝体的致死温度为50℃处理10分钟。此外，99.1%丙环唑和98.7%嘧菌酯抑菌效果最佳，抑菌率为50%时的浓度分别为16.85和12.24μg·mL-1。基于以上结果，福建省导致太子参紫纹羽病的病原菌为桑卷担菌。培养基类型、生长温度、pH值、氮源等对菌丝体生长有显著影响。
