State Key Laboratory of Agricultural Microbiology, Wuhan, Hubei, 430070, P.R. China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China.
Sci Rep. 2017 Sep 12;7(1):11329. doi: 10.1038/s41598-017-11743-7.
A disease causing smaller and cracked fruit affects peach [Prunus persica (L.) Batsch], resulting in significant decreases in yield and quality. In this study, peach tree leaves showing typical symptoms were subjected to deep sequencing of small RNAs for a complete survey of presumed causal viral pathogens. The results revealed two known viroids (Hop stunt viroid and Peach latent mosaic viroid), two known viruses (Apple chlorotic leaf spot trichovirus and Plum bark necrosis stem pitting-associated virus) and a novel virus provisionally named Peach leaf pitting-associated virus (PLPaV). Phylogenetic analysis based on RNA-dependent RNA polymerase placed PLPaV into a separate cluster under the genus Fabavirus in the family Secoviridae. The genome consists of two positive-sense single-stranded RNAs, i.e., RNA1 [6,357 nt, with a 48-nt poly(A) tail] and RNA2 [3,862 nt, with a 25-nt poly(A) containing two cytosines]. Biological tests of GF305 peach indicator seedlings indicated a leaf-pitting symptom rather than the smaller and cracked fruit symptoms related to virus and viroid infection. To our knowledge, this is the first report of a fabavirus infecting peach. PLPaV presents several new molecular and biological features that are absent in other fabaviruses, contributing to an overall better understanding of fabaviruses.
一种导致果实变小和开裂的病害影响桃[Prunus persica (L.) Batsch]，导致产量和质量显著下降。在这项研究中，对表现出典型症状的桃树叶片进行了小 RNA 的深度测序，以全面调查疑似致病的病毒病原体。结果显示了两种已知的类病毒（Hop stunt 类病毒和桃潜隐花叶类病毒）、两种已知的病毒（苹果褪绿斑卷叶病毒和李树皮坏死茎痘相关病毒）和一种新的病毒，暂命名为桃叶片痘相关病毒（PLPaV）。基于 RNA 依赖的 RNA 聚合酶的系统发育分析将 PLPaV 置于正粘病毒科细环病毒属的一个单独聚类中。基因组由两条正链单链 RNA 组成，即 RNA1[6,357nt，带有 48nt 的 poly(A)尾]和 RNA2[3,862nt，带有 25nt 的 poly(A)，包含两个胞嘧啶]。对 GF305 桃指示幼苗的生物学测试表明出现叶片痘症状，而不是与病毒和类病毒感染相关的果实变小和开裂症状。据我们所知，这是首次报道类病毒感染桃。PLPaV 具有几种新的分子和生物学特征，在其他类病毒中不存在，有助于更好地了解类病毒。
