Wang Yabo, Liu Xinxin
Department of Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
Department of Geriatrics, First Affiliated Hospital of Henan University, Kaifeng 475000, China.
Evid Based Complement Alternat Med. 2023 Jan 23;2023:9935942. doi: 10.1155/2023/9935942. eCollection 2023.
C. A. Mey () is a traditional Chinese medicinal herb used for the treatment of nervous system disorders, such as Alzheimer's disease (AD). However, the pharmacological mechanisms of involved in AD have not been systematically investigated. Here, a network pharmacology approach was adopted to explore the effective components, core targets, and key pathways of against AD.
TCMSP database was used to screen the active ingredients of . Prediction of the targets of ginseng and AD-related genes was performed using online public databases. "Compound-Target," "Compound-Target-Disease," "Protein-Protein Interaction (PPI)," "Compound-Target-Pathway," and "Compound-Target-GO-Pathway" networks were constructed with Cytoscape 3.7.2 software. Gene Ontology (GO) function annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed by using the DAVID database.
A total of 22 bioactive compounds were identified from , and 481 targets of and 763 AD-related targets were obtained from public databases. The PPI network screened out 19 hub genes of against AD. According to GO function enrichment, ginseng influenced cell proliferation, death, the nitric oxide biosynthetic process, hypoxia response, and synaptic transmission. Neuroactive ligand-receptor interaction, serotonergic synapse, calcium signaling, cAMP signaling, FoxO signaling, Ras signaling, and PI3K-AKT signaling were among the most key regulatory pathways. The compound-target-GO-route network found EGFR, MAPK1, MAPK14, AKT1, CASP3, and PRKACA as key genes, with PI3K-AKT signaling being the most important pathway for ginseng's anti-AD activity.
exerts neuroprotective effects in AD patients through multicomponent, multitarget, and multipathway modes, providing novel insight into the pharmacological and experimental research on against AD.
刺五加是一种用于治疗神经系统疾病（如阿尔茨海默病（AD））的传统中草药。然而，其在AD中的药理机制尚未得到系统研究。在此，采用网络药理学方法探索刺五加抗AD的有效成分、核心靶点和关键途径。
利用中药系统药理学数据库与分析平台（TCMSP）筛选刺五加的活性成分。使用在线公共数据库对人参靶点和AD相关基因进行预测。用Cytoscape 3.7.2软件构建“化合物-靶点”“化合物-靶点-疾病”“蛋白质-蛋白质相互作用（PPI）”“化合物-靶点-途径”和“化合物-靶点-GO-途径”网络。利用DAVID数据库进行基因本体（GO）功能注释和京都基因与基因组百科全书（KEGG）途径富集分析。
从刺五加中鉴定出22种生物活性化合物，从公共数据库中获得刺五加的481个靶点和763个AD相关靶点。PPI网络筛选出刺五加抗AD的19个枢纽基因。根据GO功能富集分析，人参影响细胞增殖、死亡、一氧化氮生物合成过程、缺氧反应和突触传递。神经活性配体-受体相互作用、5-羟色胺能突触、钙信号、环磷酸腺苷（cAMP）信号、叉头框蛋白O（FoxO）信号、Ras信号和磷脂酰肌醇-3激酶-蛋白激酶B（PI3K-AKT）信号是最关键的调控途径。化合物-靶点-GO-途径网络发现表皮生长因子受体（EGFR）、丝裂原活化蛋白激酶1（MAPK1）、丝裂原活化蛋白激酶14（MAPK14）、蛋白激酶B（AKT1）、半胱天冬酶3（CASP3）和蛋白激酶A（PRKACA）为关键基因，PI3K-AKT信号是人参抗AD活性最重要的途径。
刺五加通过多成分、多靶点和多途径模式对AD患者发挥神经保护作用，为刺五加抗AD的药理及实验研究提供了新的见解。
