Eid Refaat A, Eldeen Muhammad Alaa, Soltan Mohamed A, Al-Shraim Mubarak, Aldehri Majed, Alqahtani Leena S, Alsharif Ghadi, Albogami Sarah, Jafri Ibrahim, Fayad Eman, Park Moon Nyeo, Bibi Shabana, Behairy Mohammed Y, Kim Bonglee, Zaki Mohamed Samir A
Pathology Department, College of Medicine, King Khalid University, Abha, Saudi Arabia.
Cell Biology, Histology & Genetics Division, Biology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Front Genet. 2023 Jan 16;13:1008502. doi: 10.3389/fgene.2022.1008502. eCollection 2022.
: Mammalian WD-repeat protein 12 (WDR12), a family member of proteins containing repeats of tryptophan-aspartic acid (WD), is a potential homolog of yeast Ytm1p and consists of seven repeats of WD. : This study aims to investigate the potential oncogenic effects of WDR12 in various human malignancies throughout a pan-cancer analysis that has been carried out to examine the various patterns in which this gene is expressed and behaves in tumor tissues. : Herein, we used The Cancer Genome Atlas (TCGA) and various computational tools to explore expression profiles, prognostic relevance, genetic mutations, immune cell infiltration, as well as the functional characteristics of WDR12 in multiple human cancers. : We found that WDR12 was inconsistently expressed in various cancers and that variations in WDR12 expression predicted survival consequences for cancer patients. Furthermore, we observed a significant correlation between WDR12 gene mutation levels and the prognosis of some tumors. Furthermore, significant correlations were found between WDR12 expression patterns and cancer-associated fibroblast (CAF) infiltration, myeloid-derived suppressor cells (MDSCs), tumor mutation burden, microsatellite instability and immunoregulators. Ultimately, pathway enrichment analysis revealed that WDR12-related pathways are involved in carcinogenesis. : The findings of our study are stisfactory, demonstrating that WDR12 could serve as a promising reliable prognostic biomarker, as well as a therapeutic target for novel cancer therapeutic approaches.
哺乳动物WD重复蛋白12（WDR12）是一种含有色氨酸-天冬氨酸（WD）重复序列的蛋白质家族成员，是酵母Ytm1p的潜在同源物，由七个WD重复序列组成。本研究旨在通过全癌分析来探究WDR12在各种人类恶性肿瘤中的潜在致癌作用，该分析旨在研究该基因在肿瘤组织中的表达和行为模式。在此，我们使用癌症基因组图谱（TCGA）和各种计算工具来探索WDR12在多种人类癌症中的表达谱、预后相关性、基因突变、免疫细胞浸润以及功能特征。我们发现WDR12在各种癌症中的表达不一致，并且WDR12表达的变化预示着癌症患者的生存结果。此外，我们观察到WDR12基因突变水平与某些肿瘤的预后之间存在显著相关性。此外，还发现WDR12表达模式与癌症相关成纤维细胞（CAF）浸润、髓源性抑制细胞（MDSC）、肿瘤突变负荷、微卫星不稳定性和免疫调节因子之间存在显著相关性。最终，通路富集分析表明WDR12相关通路参与了致癌过程。我们的研究结果令人满意，表明WDR12可作为一个有前景的可靠预后生物标志物，以及新型癌症治疗方法的治疗靶点。
