12周联合训练对非酒精性脂肪性肝病患者肝脏脂肪含量和代谢灵活性的影响：一项开放标签、单中心随机对照试验方案
The effect of 12 weeks of combined training on hepatic fat content and metabolic flexibility of individuals with non-alcoholic fatty liver disease: Protocol of an open-label, single-center randomized control trial.
作者信息
Huang Wei, Ruan Weiqi, Huo Cuilan, Lin Yanyu, Wang Tian, Dai Xiangdi, Zhai Haonan, Ma Jiasheng, Zhang Jingyi, Lu Jin, Zhuang Jie
机构信息
Shanghai Frontiers Science Research Base of Exercise and Metabolic Health, Shanghai University of Sport, Shanghai, China.
School of Exercise and Health, Shanghai University of Sport, Shanghai, China.
出版信息
Front Nutr. 2023 Jan 16;9:1065188. doi: 10.3389/fnut.2022.1065188. eCollection 2022.
INTRODUCTION
Metabolic flexibility (MetF) is the capacity of an organism to oxidate substrate according to substrate availability or demand. The mismatch of substrate availability and oxidation may cause ectopic fat accumulation in the muscle and the liver. The objectives of the study are to examine the effect of 12 weeks of combined exercise on hepatic fat reduction and investigate metabolites related to MetF before and after the high-fat diet between individuals with NAFLD and healthy control with an active lifestyle.
METHODS
This study is an open-label, single-center trial randomized controlled clinical study plus a cross-sectional comparison between individuals with NAFLD and healthy control. Individuals with NAFLD were allocated into two groups receiving resistance training (RT) combined with high-intensity interval training (HIIT) or moderate-intensity continuous training (MICT). Anthropometric indicators, clinical blood markers about glucose, lipid metabolism, and hepatic fat content (HFC) were assessed before and after the intervention. The metabolomics was also used to investigate the discrepant metabolites and mechanisms related to MetF.
DISCUSSION
Metabolic flexibility reflects the capacity of an organism to switch the oxidation substrates flexibly, which is associated with ectopic fat accumulation. Our study aimed to explore the discrepant metabolites related to MetF before and after a high-fat diet between individuals with NAFLD and healthy control. In addition, the study also examined the effectiveness of RT combined with HIIT or MICT on hepatic fat reduction and quantificationally analyzed the metabolites related to MetF before and after the intervention. Our results provided a perspective on fatty liver-associated metabolic inactivity.
TRIAL REGISTRATION
ClinicalTrials.gov: ChiCTR2200055110; Registered 31 December 2021, http://www.chictr.org.cn/index.aspx.
引言
代谢灵活性（MetF）是生物体根据底物可用性或需求氧化底物的能力。底物可用性与氧化之间的不匹配可能导致肌肉和肝脏中异位脂肪堆积。本研究的目的是检验12周联合运动对减少肝脏脂肪的影响，并调查非酒精性脂肪性肝病（NAFLD）患者和积极生活方式的健康对照者在高脂饮食前后与代谢灵活性相关的代谢物。
方法
本研究是一项开放标签、单中心试验随机对照临床研究，外加NAFLD患者与健康对照者之间的横断面比较。将NAFLD患者分为两组，分别接受阻力训练（RT）联合高强度间歇训练（HIIT）或中等强度持续训练（MICT）。在干预前后评估人体测量指标、有关葡萄糖、脂质代谢的临床血液标志物以及肝脏脂肪含量（HFC）。代谢组学也用于研究与代谢灵活性相关的差异代谢物和机制。
讨论
代谢灵活性反映了生物体灵活切换氧化底物的能力，这与异位脂肪堆积有关。我们的研究旨在探索NAFLD患者和健康对照者在高脂饮食前后与代谢灵活性相关的差异代谢物。此外，该研究还检验了RT联合HIIT或MICT对减少肝脏脂肪的有效性，并对干预前后与代谢灵活性相关的代谢物进行了定量分析。我们的结果为脂肪肝相关的代谢不活跃提供了一个视角。
试验注册
ClinicalTrials.gov：ChiCTR2200055110；于2021年12月31日注册，http://www.chictr.org.cn/index.aspx。
Zotero 插件