Maru Viral, Kb Ashwini, Madkaikar Manisha, Devi Rk Sarada, Gada Ashita, Bapat Salil
Pediatric Dentistry, Government Dental College and Hospital, Mumbai, IND.
Dentistry, Bangalore Institute of Dental Sciences & Research Centre, Bangalore, IND.
Cureus. 2022 Dec 27;14(12):e33024. doi: 10.7759/cureus.33024. eCollection 2022 Dec.
Objectives Previous research has established that sodium hypochlorite (NaOCl) has a detrimental effect on dental stem cell viability and maturation. However, a review of the literature revealed no study evaluating the response of NaOCl to the survival of stem cells derived from human exfoliated deciduous teeth (SHEDs). Hence, the aim of the present trial was to assess the influence of various dilutions of NaOCl on SHED proliferation and differentiation. Materials and Methods The 5.25% NaOCl solutions at concentrations of 0.5, 0.1, 0.025, 0.0125, and 0.005 mg/ml were used to assess the response to SHED proliferation and differentiation through methyl tetrazolium (MTT) assay, alkaline phosphatase (ALP) assay, and reverse transcription polymerase chain reaction gene expression analysis at various time point intervals. Results MTT assay showed that the viability of SHEDs decreased with an increase in the concentration of NaOCl and an increase in incubation time. The ALP activity decreased with an increase in the concentration of NaOCl up to 14 days of incubation. However, the ALP activity of all the test specimens further decreased after 14 days of incubation. The gene expression levels of osteocalcin, dentin sialophosphoprotein, and STRO-1 were statistically significant when compared to the control after one, three, and seven days of incubation. Conclusion Different doses of NaOCl other than 0.5 mg/ml revealed encouraging outcomes in terms of proliferation, long-term ALP functioning, and odontogenic differentiation potential when cultivated in SHEDs.
目的 以往研究表明，次氯酸钠（NaOCl）对牙干细胞的活力和成熟有不利影响。然而，文献综述显示，尚无研究评估NaOCl对人乳牙脱落干细胞（SHEDs）存活的反应。因此，本试验的目的是评估不同稀释度的NaOCl对SHEDs增殖和分化的影响。材料与方法 使用浓度为0.5、0.1、0.025、0.0125和0.005 mg/ml的5.25% NaOCl溶液，通过甲基噻唑基四唑（MTT）法、碱性磷酸酶（ALP）法以及在不同时间点间隔进行逆转录聚合酶链反应基因表达分析，来评估对SHEDs增殖和分化的反应。结果 MTT法显示，SHEDs的活力随着NaOCl浓度的增加和孵育时间的延长而降低。在孵育14天内，ALP活性随着NaOCl浓度的增加而降低。然而，孵育14天后，所有测试样本的ALP活性进一步降低。与对照组相比，孵育1、3和7天后，骨钙素、牙本质涎磷蛋白和STRO-1的基因表达水平具有统计学意义。结论 当在SHEDs中培养时，除0.5 mg/ml外的不同剂量的NaOCl在增殖、长期ALP功能和牙源性分化潜能方面显示出令人鼓舞的结果。
