Marr Kendra D, Ignatenko Natalia A, Warfel Noel A, Batai Ken, Cress Anne E, Pollock Grant R, Wong Ava C, Lee Benjamin R
Cancer Biology, University of Arizona, Tucson, AZ, United States.
MD/PhD Program, College of Medicine Tucson, University of Arizona, Tucson, AZ, United States.
Front Oncol. 2023 Jan 13;12:1083150. doi: 10.3389/fonc.2022.1083150. eCollection 2022.
The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate time-lapse imaging distinct phenotypes of prostate organoids derived from patient material- without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p<0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology.
源自患者组织的永生化活类器官的出现是癌症研究的一个有前景的途径，但受到精确表征困难的限制。在这篇简短的通讯中，我们展示了延时成像技术下源自患者材料的前列腺类器官的不同表型——未对细胞身份进行确认。我们发现，源自组织学正常组织的类器官在生理性细胞外基质（ECM）上比在病理性ECM上更容易扩散（p<0.0001），而肿瘤衍生的类器官在两种基质上的扩散情况相同（p=0.2406）。这项研究是一个重要的概念验证，即推迟对类器官的精确表征，仍能收集到有关疾病病理学的信息。
