Knöller J, Schönfeld W, Bremm K D, König W
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr Universität, Bochum.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):176-81. doi: 10.1016/s0176-6724(87)80164-5.
A rapid and sensitive HPLC-method has been developed for the determination of serum concentrations of aspoxicillin (TA-058), a new semisynthetic beta-lactam antibiotic. Aspoxicillin was chromatographed with a phosphate buffer/methanol (92:8 v/v) mobile phase and a C-18 reversed phase column and was detected at a wavelength of 220 nm. The stability of aspoxicillin in serum and buffer at different temperatures was studied over a time period of 3 months. Furthermore, the degradation of aspoxicillin versus piperacillin was determined in serum and buffer at 37 degrees C. Aspoxicillin remains stable only at -70 degrees C whereas degradation has been observed at -20 degrees C and 4 degrees C. At 37 degrees C, 20% of aspoxicillin is degraded in serum within 24 h whereas piperacillin is completely degraded under the same conditions.
已开发出一种快速灵敏的高效液相色谱法，用于测定新型半合成β-内酰胺抗生素阿扑西林（TA-058）的血清浓度。阿扑西林采用磷酸盐缓冲液/甲醇（92:8 v/v）流动相和C-18反相柱进行色谱分离，并在220 nm波长处检测。在3个月的时间内研究了阿扑西林在不同温度下于血清和缓冲液中的稳定性。此外，还测定了在37℃下血清和缓冲液中阿扑西林与哌拉西林的降解情况。阿扑西林仅在-70℃时保持稳定，而在-20℃和4℃时已观察到降解。在37℃时，24小时内血清中20%的阿扑西林会降解，而哌拉西林在相同条件下会完全降解。
