Nishi H, Fukuyama T, Matsuo M
Analytical Chemistry Research Laboratory, Tanabe Seiyaku, Co., Ltd., Osaka, Japan.
J Chromatogr. 1990 Aug 31;515:245-55. doi: 10.1016/s0021-9673(01)89319-5.
Both the separation and determination of aspoxicillin in human plasma by micellar electrokinetic chromatography (MEKC) were investigated. Selectivity in the separation of seven penicillin antibiotics was improved by using MEKC in comparison with capillary zone electrophoresis. Plasma proteins, which might interfere with drug analysis in conventional chromatography, were solubilized by the micelles employed in MEKC and eluted later than the drugs. This permitted the determination of the drugs in plasma by a direct sample injection method. One analysis of a plasma sample was performed within ca. 20 min without pretreatment. Good linearity and recovery were also obtained in the range of plasma levels usually encountered in clinical analysis with a correlation coefficient r = 0.999 and 94-104% recovery. The limit of detection for aspoxicillin was 1.3 micrograms ml-1 at a signal-to-noise ratio of 3.
研究了胶束电动色谱法（MEKC）用于人血浆中阿扑西林的分离与测定。与毛细管区带电泳相比，使用MEKC提高了七种青霉素抗生素分离的选择性。在传统色谱法中可能干扰药物分析的血浆蛋白，被MEKC中使用的胶束溶解，并比药物洗脱得更晚。这使得通过直接进样法测定血浆中的药物成为可能。无需预处理，约20分钟内即可完成一份血浆样品的分析。在临床分析中通常遇到的血浆水平范围内，也获得了良好的线性和回收率，相关系数r = 0.999，回收率为94 - 104%。阿扑西林的检测限为1.3微克/毫升，信噪比为3。
