Wang Tingyin, Zhu Youzhi, Weng Shuyun, Lin Xueliang, Kong Kien Voon, Weng Youliang, Jia Xianggang, Chen Rong, Lin Duo, Feng Shangyuan
Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.
These authors contributed equally to this work.
Biomed Opt Express. 2022 Oct 20;13(11):5962-5970. doi: 10.1364/BOE.474273. eCollection 2022 Nov 1.
Monitoring the levels of cancer biomarkers is essential for cancer diagnosis and evaluation. In this study, a novel sandwich type sensing platform based on surface-enhanced Raman scattering (SERS) technology was developed for the detection of carcinoembryonic antigen (CEA), with a limit of detection (LOD) of 0.258 ng/mL. In order to achieve sensitive detection of CEA in complex samples, gold nanoparticle monolayer modified with CEA antibodies and with aptamer-functionalized probes was fabricated to target CEA. Two gold layers were integrated into the SERS platform, which greatly enhanced the signal of the probe by generating tremendous "hot spots". Meanwhile, the intensity ratio of Raman probes and the second-order peak of the silicon wafer was used to achieve dynamic calibration of the Raman probe signal. Excitingly, this sensing platform was capable of distinguishing cancer patients from healthy individuals via CEA concentrations in blood samples with the accuracy of 100%. This sandwich structure SERS sensing platform presented promising potential to be an alternative tool for clinical biomarker detection in the field of cancer diagnosis.
监测癌症生物标志物的水平对于癌症诊断和评估至关重要。在本研究中，基于表面增强拉曼散射（SERS）技术开发了一种新型夹心型传感平台，用于检测癌胚抗原（CEA），检测限（LOD）为0.258 ng/mL。为了在复杂样品中实现对CEA的灵敏检测，制备了用CEA抗体修饰且带有适体功能化探针的金纳米颗粒单层来靶向CEA。将两个金层集成到SERS平台中，通过产生大量“热点”极大地增强了探针信号。同时，利用拉曼探针与硅片二阶峰的强度比实现拉曼探针信号的动态校准。令人兴奋的是，该传感平台能够通过血液样本中的CEA浓度将癌症患者与健康个体区分开来，准确率达100%。这种夹心结构的SERS传感平台在癌症诊断领域作为临床生物标志物检测的替代工具具有广阔的应用潜力。
