右美托咪定对 X 射线诱导的睾丸损伤的放射防护作用。

Radioprotective effects of dexmedetomidine on X-ray-induced testicular damage.

机构信息

Department of Urology, Department of Histology and Embryology, Department of Radiation Oncology, Department of Biochemistry, Department of Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.

出版信息

Eur Rev Med Pharmacol Sci. 2023 Jan;27(2):673-680. doi: 10.26355/eurrev_202301_31069.

DOI:10.26355/eurrev_202301_31069

PMID:36734735

Abstract

OBJECTIVE

Approximately 70% of cancer patients require radiotherapy. However, despite its effectiveness in the treatment of cancer, radiotherapy can also affect and damage surrounding healthy tissues in addition to tumorous tissues. Since testicular tissues are highly radiosensitive, radiotherapy can cause impairments in spermatogenesis leading to infertility. The purpose of this study was to examine the potential radio-protective effect of dexmedetomidine (Dex), an α2-adrenoceptor agonist, on oxidative stress and apoptosis in testicular tissues caused by x-irradiation in rats.

MATERIALS AND METHODS

Thirty male Sprague-Dawley rats were allocated into three groups of ten (n=10): control, irradiation (IR), and IR + Dex groups. The IR group was exposed to a single dose of 2 Gy IR. The IR+Dex group was given a single intraperitoneal (i.p.) dose of 100 µg/kg Dex before IR. The control group received a single dose of saline solution i.p. Testicular tissues removed 24 hours after IR were subjected to histochemical, biochemical, and immunohistochemical analysis.

RESULTS

IR resulted in increased malondialdehyde (MDA) activity and significant changes in testis tissues. However, the application of Dex elevated glutathione levels by preventing MDA formation. In addition, Dex decreased tubular epithelial apoptosis via elevated Cleaved Caspase-3 expressions.

CONCLUSIONS

Dex exhibited a radio-protective effect against lipid peroxidation and apoptosis caused by IR.

摘要

目的

约 70%的癌症患者需要接受放疗。然而，尽管放疗在癌症治疗中具有有效性，但它也会对肿瘤组织以外的周围健康组织造成影响和损伤。由于睾丸组织对放射线高度敏感，放疗可能导致精子发生受损，从而导致不育。本研究旨在探讨α2-肾上腺素受体激动剂右美托咪定（Dex）对大鼠 X 射线照射引起的睾丸组织氧化应激和细胞凋亡的潜在保护作用。

材料和方法

将 30 只雄性 Sprague-Dawley 大鼠随机分为三组，每组 10 只（n=10）：对照组、照射组（IR）和 IR+Dex 组。IR 组接受单次 2 Gy IR。IR+Dex 组在 IR 前给予单次腹腔内（i.p.）100 µg/kg Dex。对照组给予单次腹腔内生理盐水溶液。IR 后 24 小时取出睾丸组织进行组织化学、生化和免疫组织化学分析。