Faculty of Medicine, Department of Pharmacology, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Faculty of Medicine, Department of Internal Medicine, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Naunyn Schmiedebergs Arch Pharmacol. 2019 Feb;392(2):147-157. doi: 10.1007/s00210-018-1581-6. Epub 2018 Nov 13.
According to data issued by the International Agency for Research on Cancer in 2012, the estimated number of new cases of all types of cancer worldwide was estimated to exceed 10 million, and 6 million of whom receive radiotherapy. Radiotherapy is the treatment of cancer using ionizing radiation. Our study investigated the effects of x-radiation resulting from radiotherapy (RT) on the testis at the molecular level, and prospectively considered the potential protective characteristics of antioxidants against testicular damage resulting from x-radiation. Forty male Sprague Dawley rats were allocated into five groups, control (group 1), abdominopelvic region 2-Gy-ionizing radiation (group 2), whole-body 6-Gy irradiation (group 3), 2 Gy abdominopelvic region irradiation and 300 mg/kg NAC treatment (group 4), and 6-Gy whole-body irradiation and 300 mg/kg NAC treatment (group 5). Disorganization and vacuolization were observed in the epithelial layer in atrophic seminiferous tubules in the only ionizing radiation (IR) groups. In addition, Johnsen's score decreased in the only IR groups, while testis tissue malondialdehyde (MDA) and glutathione (GSH) tissue levels increased. N-Acetylcysteine (NAC) treatment groups Johnsen's score and tissue GSH levels increased than only IR groups. On the other hand, tissue MDA levels decreased in the NAC treatment groups. The findings showed that ionizing radiation caused apoptosis in germinal epithelial cells led to the oxidative stress-mediated testicular injury. On the other hand, NAC may be useful in the prevention of testicular injury-suppressed ROS production.
根据国际癌症研究机构 2012 年发布的数据，全球预计新增癌症病例超过 1000 万例，其中 600 万人接受放疗。放射疗法是利用电离辐射治疗癌症。我们的研究从分子水平上调查了放疗（RT）引起的 X 射线对睾丸的影响，并前瞻性地考虑了抗氧化剂对 X 射线引起的睾丸损伤的潜在保护特性。将 40 只雄性 Sprague Dawley 大鼠分配到 5 组中：对照组（第 1 组）、腹部和骨盆 2-Gy 电离辐射（第 2 组）、全身 6-Gy 照射（第 3 组）、2 Gy 腹部和骨盆照射和 300mg/kg NAC 治疗（第 4 组），以及 6-Gy 全身照射和 300mg/kg NAC 治疗（第 5 组）。只有电离辐射（IR）组的曲细精管上皮层出现了结构紊乱和空泡化。此外，只有 IR 组的 Johnsen 评分降低，而睾丸组织丙二醛（MDA）和谷胱甘肽（GSH）组织水平升高。N-乙酰半胱氨酸（NAC）治疗组的 Johnsen 评分和组织 GSH 水平高于只有 IR 组。另一方面，NAC 治疗组的组织 MDA 水平降低。研究结果表明，电离辐射导致生殖上皮细胞凋亡，导致氧化应激介导的睾丸损伤。另一方面，NAC 可能有助于抑制 ROS 产生，从而预防睾丸损伤。
