Department of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, People's Republic of China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, People's Republic of China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haidian District, Beijing 100193, PR. China.
Int J Med Mushrooms. 2023;25(1):65-76. doi: 10.1615/IntJMedMushrooms.2022046594.
As a wood-degrading Agaricomycetes mushroom, Ganoderma lucidum can be cultivated on broad-leaf hardwoods. Generally, producers care about the yield, but not the quality of G. lucidum cultivated by different tree species. In this study, five broad-leaf hardwood tree species-Quercus variabilis Bl. (Qv), Castanea mollissima Bl. (Cm), Liquidambar formosana Hance (Lf), Dalbergia hupeana Hance (Dh), and Platycarya strobilacea Sieb. et Zucc. (Ps)-were selected for cultivating of G. lucidum. The chemical compositions of G. lucidum fruiting bodies produced by these tree species were determined by Fourier transform infrared and two-dimensional infrared correlation spectroscopy in order to select the most suitable tree species for cultivation. The overall spectra showed less discrimination of each peak variation detected and properly kept most of the primary metabolites. The second derivative unfolded the stagnation of the first spectrum and more base peaks were detected especially in the range of the first two sections. The protein content contained in G. lucidum cultivated on Ps was 92%, like that on Dh. On the other hand, only 27% similarity was determined in G. lucidum cultivated on Ps and Qv. Therefore, the correlation of this range for the protein content can help in tree species selection. The active sequence of 2DIR spectral could be determined by the active bonding of the component reacted to the perturbation. The result could provide a scientific basis for the selection of tree species and the comprehensive utilization of broad-leaf tree resources on G. lucidum cultivation.
作为一种木质腐朽的伞菌，灵芝可以在阔叶硬木上栽培。一般来说，生产者关心的是产量，而不是不同树种栽培灵芝的质量。在这项研究中，选择了 5 种阔叶硬木树种，即麻栎（Qv）、板栗（Cm）、枫香（Lf）、黄檀（Dh）和山核桃（Ps）来栽培灵芝。采用傅里叶变换红外和二维相关光谱技术对这些树种产生的灵芝子实体的化学成分进行了测定，以选择最适合栽培的树种。总体光谱显示，每个峰变检测的区分度较低，并且适当保留了大多数初级代谢物。二阶导数展开了第一谱的停滞，并且检测到更多的基本峰，特别是在前两个部分的范围内。在 Ps 和 Dh 上栽培的灵芝中的蛋白质含量为 92%，与 Dh 上的含量相同。另一方面，在 Ps 和 Qv 上栽培的灵芝中只确定了 27%的相似性。因此，该范围内的相关性可以帮助选择树种。2DIR 光谱的活性序列可以通过与扰动反应的组分的活性键来确定。研究结果可为灵芝栽培中树种选择和阔叶树资源综合利用提供科学依据。
