School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, P.R. China.
Key State-owned Forest Technical Service Center of Jilin Province, Changchun 130022, P.R. China.
Int J Med Mushrooms. 2023;25(6):87-98. doi: 10.1615/IntJMedMushrooms.2023048272.
To investigate the differences between Korean Ganoderma lucidum spore powder (KP), broken-spo-roderm KP (BSKP), Chinese traditional G. lucidum spore powder (CP), and broken-sporoderm CP (BSCP), they were identified by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), second derivative infrared spectroscopy (SD-IR), dual-index sequence analysis (DISA) and X-ray diffraction (XRD). SEM showed that there were no significant differences in microstructure between the two kinds of spore powders. FT-IR spectra showed that the four spore powders appeared with characteristic peaks of 3400, 3006, 2925, 1745, 1535, 1454, 1249, 1074, 1049, and 896 cm-1, respectively, they were contained the characteristic peaks of total triterpenes, polysaccharides and fatty acids. DISA showed that the same species of spore powders, the overall similarity of before and broken the sporoderm was high with minor differences and there were no differences between the different kinds of spore powders. Similarity analysis showed that the four spore powders were in high agreement and were no differences. The polysaccharide, total triterpene, spore oil and protein content of the four spore powders were determined separately. The results showed that the active ingredients content of the batch of KP were lower than that of CP, that of BSKP were lower than that of BSCP, while the active ingredients content of both broken-sporoderm spore powders were higher than that of before broken-sporoderm. It is inferred that the structure of the main chemical and component of KP is the same as that of CP. This study provides a reference for the future development and application of G. lucidum.
为了研究韩国灵芝孢子粉（KP）、破壁灵芝孢子粉（BSKP）、中国传统灵芝孢子粉（CP）和破壁灵芝孢子粉（BSCP）之间的差异，我们采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、二阶导数红外光谱（SD-IR）、双索引序列分析（DISA）和 X 射线衍射（XRD）对它们进行了鉴定。SEM 显示，两种孢子粉的微观结构没有明显差异。FT-IR 光谱显示，四种孢子粉均出现 3400、3006、2925、1745、1535、1454、1249、1074、1049 和 896 cm-1 的特征峰，分别含有总三萜、多糖和脂肪酸的特征峰。DISA 显示，同一种孢子粉，破壁前后的整体相似性较高，只有细微差异，不同种类的孢子粉之间没有差异。相似性分析表明，四种孢子粉高度一致，没有差异。分别测定了四种孢子粉的多糖、总三萜、孢子油和蛋白质含量。结果表明，KP 批的活性成分含量低于 CP，BSKP 的活性成分含量低于 BSCP，而两种破壁孢子粉的活性成分含量均高于破壁前。可以推断 KP 的主要化学成分和结构与 CP 相同。本研究为灵芝的未来开发和应用提供了参考。
