Department of Histology and Embryology, School of Medicine, Gaziantep University, Gaziantep, Turkey.
Department of Histology and Embryology, Aydın Adnan Menderes University, Aydın, Turkey.
Biol Trace Elem Res. 2023 Oct;201(10):4834-4849. doi: 10.1007/s12011-023-03565-8. Epub 2023 Feb 3.
Liver fibrosis is a worldwide public health problem due to its life-threatening complications, including portal hypertension, liver failure, cirrhosis, and hepatocellular carcinoma (HCC). Liver fibrosis is the net result of a complex excessive accumulation of extracellular matrix (ECM). Activation of hepatic stellate cells (HSCs) are the cause of deposition of ECM and are commonly recognized as a key step in liver fibrosis. The aim of this study was to investigate the effect of foreskin-derived mesenchymal stem cells treated with boron compounds on liver fibrosis. Rats were injected intraperitoneally with thioacetamide (TAA) at a dose of 150 mg/kg except sham and control groups' rats. Thioacetamide (TAA), foreskin-derived mesenchymal stem cells (TAA + FSDMSC), FSDMSC treated with boric acid (TAA + FSDMSC + BA), FSDMSC treated with sodium pentaborate pentahydrate (TAA + FSDMSC + NaB), control and sham groups were studied. Boron compound treated foreskin-derived mesenchymal stem cells were injected into the tail vein, and evaluations were conducted after 4 weeks and liver tissues were obtained for structural, immunohistochemical, and western blot studies and blood samples were taken for biochemical analysis. FSDMSC (BA) alleviates TAA-induced rats liver fibrosis, and BA showed a positive effect on foreskin-derived mesenchymal stem cells viability. After using BA-treated mesenchymal stem cells, we observed that there was regression in the fibrotic areas at TAA-induced liver fibrosis. The result demonstrates that the contribution of TAA + FSDMSC and TAA + FSDMSC (NaB) at the level of structure is not effective in regression of fibrosis in TAA-generated liver fibrosis. We concluded that FSDMSC treated with BA may be a factor in the regression of fibrosis.
肝纤维化是一个全球性的公共卫生问题，因为它可能导致危及生命的并发症，包括门静脉高压、肝功能衰竭、肝硬化和肝细胞癌（HCC）。肝纤维化是细胞外基质（ECM）过度积累的净结果。肝星状细胞（HSCs）的激活是 ECM 沉积的原因，通常被认为是肝纤维化的关键步骤。本研究旨在探讨硼化合物处理的包皮来源间充质干细胞对肝纤维化的影响。除假手术和对照组大鼠外，其余大鼠均腹腔注射硫代乙酰胺（TAA），剂量为 150mg/kg。研究了 TAA（TAA+FSDMSC）、硼处理的包皮来源间充质干细胞（TAA+FSDMSC+BA）、硼酸钠处理的包皮来源间充质干细胞（TAA+FSDMSC+NaB）、对照组和假手术组。将硼化合物处理的包皮来源间充质干细胞注入尾静脉，4 周后进行评估，并获取肝组织进行结构、免疫组织化学和 Western blot 研究，采集血液样本进行生化分析。（BA）减轻 TAA 诱导的大鼠肝纤维化，BA 对包皮来源间充质干细胞活力有积极影响。在用 BA 处理后的间充质干细胞后，我们观察到 TAA 诱导的肝纤维化中纤维化区域出现了消退。结果表明，在结构水平上，TAA+FSDMSC 和 TAA+FSDMSC（NaB）的作用不能有效地逆转 TAA 诱导的肝纤维化中的纤维化。我们得出结论，BA 处理的 FSDMSC 可能是纤维化消退的一个因素。
