Sogut Ibrahim, Oglakci Aysegul, Kartkaya Kazim, Ol Kevser Kusat, Sogut Melis Savasan, Kanbak Gungor, Inal Mine Erden
Department of Medical Services and Techniques, Vocational School of Health Services, Istanbul Bilim University, Istanbul 34394, Turkey.
Department of Biochemistry, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir 26480, Turkey.
Exp Ther Med. 2015 Mar;9(3):1023-1027. doi: 10.3892/etm.2014.2164. Epub 2014 Dec 30.
To the best of our knowledge, this is the first study concerning the effect of boric acid (BA) administration on fetal alcohol syndrome (FAS). In this study, the aim was to investigate prenatal alcohol-induced oxidative stress on the cerebral cortex of newborn rat pups and assess the protective and beneficial effects of BA supplementation on rats with FAS. Pregnant rats were divided into three groups, namely the control, alcohol and alcohol + boric acid groups. As markers of alcohol-induced oxidative stress in the cerebral cortex of the newborn pups, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels were measured. Although the MDA levels in the alcohol group were significantly increased compared with those in the control group (P<0.05), the MDA level in the alcohol + boric acid group was shown to be significantly decreased compared with that in the alcohol group (P<0.01). The CAT activity of the alcohol + boric acid group was significantly higher than that in the alcohol group (P<0.05). The GPx activity in the alcohol group was decreased compared with that in the control group (P<0.05). These results demonstrate that alcohol is capable of triggering damage to membranes of the cerebral cortex of rat pups and BA could be influential in antioxidant mechanisms against oxidative stress resulting from prenatal alcohol exposure.
据我们所知，这是第一项关于硼酸（BA）给药对胎儿酒精综合征（FAS）影响的研究。在本研究中，目的是调查产前酒精诱导的新生大鼠幼崽大脑皮质氧化应激，并评估补充BA对FAS大鼠的保护和有益作用。将怀孕大鼠分为三组，即对照组、酒精组和酒精+硼酸组。测量新生幼崽大脑皮质中丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平，作为酒精诱导氧化应激的标志物。与对照组相比，酒精组的MDA水平显著升高（P<0.05），而酒精+硼酸组的MDA水平与酒精组相比显著降低（P<0.01）。酒精+硼酸组的CAT活性显著高于酒精组（P<0.05）。酒精组的GPx活性与对照组相比降低（P<0.05）。这些结果表明，酒精能够引发大鼠幼崽大脑皮质膜的损伤，而BA可能对产前酒精暴露引起的氧化应激的抗氧化机制有影响。
