单价电解质溶液对人泪液拉丝模式的影响。
Effect of monovalent electrolyte solutions on the human tear ferning pattern.
机构信息
Department of Optometry, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
出版信息
PLoS One. 2023 Feb 3;18(2):e0280853. doi: 10.1371/journal.pone.0280853. eCollection 2023.
PURPOSE
To investigate the effect of the addition of a low concentration of sodium chloride (NaCl) and potassium chloride (KCl) solutions on the tear ferning (TF) patterns of tears collected from humans.
METHODS
A tear sample (20 μL) was collected from the right eye of 23 males and 7 females (25.4 ± 6.6 years). The tears were collected in one sitting for healthy subjects (N = 13). For dry eye participants (N = 17), the tear samples were collected in two separate settings with five minutes gap in between. A sample (1 μL) from each tear was dried on a glass slide, and the obtained ferns were observed using a microscope and graded using the five-point TF grading scale. Mixtures of tear samples (0.5 μL) and different volumes (0.5-2.5 μL) of each electrolyte (10-30 mg in 100 mL of water) solution were prepared, and their TF patterns were recorded and compared with those of the corresponding pure tears.
RESULTS
Significant improvements (Wilcoxon test, P < 0.001) have been seen in the TF grades of the tear samples after the addition of NaCl and KCl solutions. A significant difference (Wilcoxon test, P = 0.016) was found between the TF grades when NaCl and KCl solutions were added to the tear samples. The TF grades of pure tears collected from dry-eye subjects ranged from 2.1 to 3.5, based on the five points grading scale, and decreased to be in the range of 0.4 to 1.6 after the addition of electrolyte solutions. While the TF grades of pure tears collected from normal-eye ranged from 1.2 to 1.9 and improved after the addition of electrolyte solution to be in the range of 0.4 to 1.5.
CONCLUSIONS
The TF test was used in vitro to assess the impact of the addition of a low concentration of sodium and potassium chloride solutions on tears collected from humans. The TF grades of human tears significantly improved after the addition of either sodium or potassium chloride solution. The mechanism for the improvement in TF grades due to the addition of electrolyte solutions must be investigated.
目的
研究低浓度氯化钠（NaCl）和氯化钾（KCl）溶液的添加对人泪液的泪液蕨类（TF）模式的影响。
方法
从 23 名男性和 7 名女性（25.4±6.6 岁）的右眼采集 20 μL 泪液样本。健康受试者（N=13）一次性采集所有泪液样本。对于干眼症患者（N=17），两次采集泪液样本之间间隔五分钟。将每个泪液样本的 1 μL 样本在载玻片上干燥，使用显微镜观察获得的蕨类并使用五点 TF 分级量表进行分级。混合泪液样本（0.5 μL）和每种电解质（在 100 mL 水中 10-30 mg）溶液的不同体积（0.5-2.5 μL），记录并比较其 TF 模式与相应纯泪液的 TF 模式。
结果
添加 NaCl 和 KCl 溶液后，泪液样本的 TF 等级明显提高（Wilcoxon 检验，P<0.001）。在向泪液样本中添加 NaCl 和 KCl 溶液时，TF 等级有显著差异（Wilcoxon 检验，P=0.016）。根据五分制评分标准，干眼症患者采集的纯泪液的 TF 等级范围为 2.1 至 3.5，添加电解质溶液后降至 0.4 至 1.6 范围。而正常眼采集的纯泪液的 TF 等级范围为 1.2 至 1.9，添加电解质溶液后提高至 0.4 至 1.5 范围。
结论
本研究采用体外 TF 试验评估低浓度氯化钠和氯化钾溶液添加对人泪液的影响。添加氯化钠或氯化钾溶液后，人泪液的 TF 等级明显提高。必须研究添加电解质溶液后 TF 等级提高的机制。
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