Abedini Mitra, Montazeri Hojatollah, Lotfali Ensieh, Ghasemi Reza, Badieyan Saeedesadat, Sasanpour Pezhman
Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Photochem Photobiol B. 2023 Mar;240:112630. doi: 10.1016/j.jphotobiol.2022.112630. Epub 2022 Dec 15.
Candidiasis occurs mainly in immunocompromised patients. Rapid detection of Candida species can play a major role in the successful management of fungal infections and treatment monitoring. Detection and discrimination of five common strains of Candida species was performed using the optical elastic scattering diffraction pattern of their colonies. Using laser light with 632 nm wavelength and the designed optical system, optical diffraction patterns of C. albicans (ATCC12261), C. tropicalis (ATCC20336), C. glabrata (15545), C. guilliermondii (20216), and C. parapsilosis (22019) were recorded, processed and analyzed. The results of our study show that based on the different structure of Candida species and dissimilar structure of their colonies, the difference between acquired diffraction patterns are recognizable. In addition, through extraction of statistical feature of the diffraction pattern images and using classification techniques, the detection and discrimination could be performed in a semi-automatic way. The analysis of the colonies of five different Candida species by the optical diffraction patterns generated from the interaction of the laser with colonies' structures demonstrated that the technique had the potential to be applied for the detection and discrimination of various species.
念珠菌病主要发生在免疫功能低下的患者中。快速检测念珠菌属在真菌感染的成功管理和治疗监测中可发挥重要作用。利用念珠菌菌落的光学弹性散射衍射图样对五种常见念珠菌菌株进行检测和鉴别。使用波长为632 nm的激光和设计的光学系统，记录、处理和分析白色念珠菌（ATCC12261）、热带念珠菌（ATCC20336）、光滑念珠菌（15545）、季也蒙念珠菌（20216）和近平滑念珠菌（22019）的光学衍射图样。我们的研究结果表明，基于念珠菌属的不同结构及其菌落的不同结构，可以识别所获得的衍射图样之间的差异。此外，通过提取衍射图样图像的统计特征并使用分类技术，可以以半自动方式进行检测和鉴别。通过激光与菌落结构相互作用产生的光学衍射图样对五种不同念珠菌的菌落进行分析表明，该技术有潜力应用于各种念珠菌的检测和鉴别。
